Genetic manipulation of giant viruses and their host,Acanthamoeba castellanii Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio Published: 2023-11-14 DOI: 10.1038/s41596-023-00910-y CRISPR-Cas9 Genetic manipulation Giant viruses Acanthamoeba castellanii Homologous recombination AI 解读 浏览 1504 PDFMetadataSupplementary information Supplementary Information Supplementary Methods Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols A dynamic DNA tetrahedron framework for active targeting BID-seq for transcriptome-wide quantitative sequencing of mRNA pseudouridine at base resolution Publisher Correction: Metagenome-assembled genome extraction and analysis from microbiomes using KBase Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology The trRosetta server for fast and accurate protein structure prediction Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish Author Correction: Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq Interrogating in vivo T-cell metabolism in mice using stable isotope labeling metabolomics and rapid cell sorting 查看全部 Pilot Exercise: Generating the Illumina SampleSheet and sharing data via BaseSpace Immunohistochemical labelling of lower urinary tract afferents in spinal cord activity-mapping neuroanatomy immunohistochemistry MIBI staining SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch Vu, M-A. T. et al (2024) Targeted micro-fiber arrays for measuring and manipulating localized multi-scale neural dynamics over large, deep brain volumes during behavior Immunoblot S1: Step-by-step-guide using image J in the work flow. Hafting tests on tanged blanks archeology experimentation archeozoology lithic knapping hafting balistics use-wear MSA Cell culture Automated concentration of viral particles in wastewater samples using Nanotrap Microbiome A Beads Nanotrap automated concentration wastewater concentration SARS-CoV-2 查看全部 ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells antibody beginner clearing expansion microscopy fluorescence microscopy HeLa cells hydrogel imaging immunocytochemistry immunohistochemistry microscopy microtubules super-resolution microscopy Testing Graphical Causal Models Using the R Package “dagitty” dagitty directed acyclic graphs (DAGs) independence testing model testing Animal Models of Osteoarthritis Part 1–Preclinical Small Animal Models: Challenges and Opportunities for Drug Development drug development guinea pig mouse osteoarthritis preclinical animal model rabbit rat zebrafish Coated Latex Beads as Artificial Cells for Quantitative Investigations of Receptor/Ligand Interactions beads flow cytometry quantification stimulation Functional Assays Evaluating Immunosuppression Mediated by Myeloid-Derived Suppressor Cells cell culture chronic inflammation flow cytometry immunosuppression inflammatory mouse model MDSC myeloid-derived suppressor cell Role of High-Throughput Electrophysiology in Drug Discovery drug discovery high-throughput electrophysiology ion channel patch clamp Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes epidermal biology induced pluripotent stem cell iPSC iPSC-derived keratinocytes keratinocytes skin diseases Downregulation of Hypoxia Inducible Factor-1α in Primary Human Natural Killer Cells Using Small Interfering RNA Delivery with ExPERT ATx by MaxCyte ExPERT ATx HIF-1α knockdown MaxCyte NK cells siRNA transfection mOTUs: Profiling Taxonomic Composition, Transcriptional Activity and Strain Populations of Microbial Communities metagenomics metatranscriptomics taxonomic profiling 查看全部