Genetic manipulation of giant viruses and their host,Acanthamoeba castellanii Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio Published: 2023-11-14 DOI: 10.1038/s41596-023-00910-y CRISPR-Cas9 Genetic manipulation Giant viruses Acanthamoeba castellanii Homologous recombination AI 解读 浏览 1249 PDFMetadataSupplementary information Supplementary Information Supplementary Methods Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Preparation of single-cell suspensions of mouse glomeruli for high-throughput analysis cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements) Genome-wide pooled CRISPR screening in neurospheres Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Design and fabrication of wearable electronic textiles using twisted fiber-based threads Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells Scanorama: integrating large and diverse single-cell transcriptomic datasets 查看全部 Image Registration of MALDI IMS to Microscopy HuBMAP BIOMIC Vanderbilt Multimodal Imaging IMS Image Registration Creating Differential Transcript Expression Results with DESeq2 DESeq2 nanopore transcript DE expression Fiber Photometry Acquisition ASAPCRN Optogenetic mouse brain Nielsen and Ford (2023) M4-mediated cholinergic transmission is reduced in parkinsonian mice and its restoration alleviates motor deficits and levodopa-induced dyskinesia ASAPCRN Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302) T7 Endonuclease I targeting efficiency PCR and gel electrophoresis Cryo-EM sample preparation for RCKW:DARPin complex cryo-EM LRRK2 structural biology ASAPCRN Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, and LPPA24S) in settled solids using digital RT-PCR SARS-CoV-2 wastewater alpha beta gamma variant mutation COVID-10 Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types miRNAs miRNA sequencing miRNA methylation miRNA profiling rare cell types mammalian system B cells Inhibitor-free DNA extraction from soil and sediment samples 查看全部 Protein Sequence Analysis Using the MPI Bioinformatics Toolkit CLANS cluster analysis HHpred HMM homology profile hidden Markov models sequence comparison sequence similarity searches structure prediction Tackling the Mouse-on-Mouse Problem in Cochlear Immunofluorescence: A Simple Double-Blocking Protocol for Immunofluorescent Labeling of Murine Cochlear Sections with Primary Mouse Antibodies antibodies cochlea histological technique immunofluorescence mouse staining Isolation and Micromass Culturing of Primary Chicken Chondroprogenitor Cells for Cartilage Regeneration cartilage formation chicken embryo chondrogenesis limb bud micromass culture Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Using CRISPR/ttLbCas12a for in planta Gene Targeting in A. thaliana CRISPR/Cas Cas12a temperature-tolerant Using ConSurf to Detect Functionally Important Regions in RNA ConSurf evolutionary analysis functional regions Rate4Site RNA sequence analysis Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology AAV localized gene therapy optogenetics single-cell biology viral vector OptoAAV technology Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis Design and Derivation of Multi-Reporter Pluripotent Stem Cell Lines via CRISPR/Cas9n-Mediated Homology-Directed Repair CRISPR/Cas9n human pluripotent stem cells reporter cell lines transfection of cells Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis 查看全部
