Genetic manipulation of giant viruses and their host,Acanthamoeba castellanii Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio Published: 2023-11-14 DOI: 10.1038/s41596-023-00910-y CRISPR-Cas9 Genetic manipulation Giant viruses Acanthamoeba castellanii Homologous recombination AI 解读 浏览 1502 PDFMetadataSupplementary information Supplementary Information Supplementary Methods Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols A dynamic DNA tetrahedron framework for active targeting An MSstats workflow for detecting differentially abundant proteins in large-scale data-independent acquisition mass spectrometry experiments with FragPipe processing BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS Tutorial: a guide for the selection of fast and accurate computational tools for the prediction of intrinsic disorder in proteins Structural characterization of protein-material interfacial interactions using lysine reactivity profiling-mass spectrometry TAC–TIC, a high-throughput genetics method to identify triggers or blockers of bacterial toxin–antitoxin systems Microbiology techniques Phage biology Prokaryote Bacteriophages Bacterial genetics Microtiter plate assays to assess antibiofilm activity against bacteria Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements) A practical guide to large-scale docking Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins 查看全部 Pilot Exercise: Generating the Illumina SampleSheet and sharing data via BaseSpace Immunohistochemical labelling of lower urinary tract afferents in spinal cord activity-mapping neuroanatomy immunohistochemistry MIBI staining SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch Vu, M-A. T. et al (2024) Targeted micro-fiber arrays for measuring and manipulating localized multi-scale neural dynamics over large, deep brain volumes during behavior Immunoblot S1: Step-by-step-guide using image J in the work flow. Hafting tests on tanged blanks archeology experimentation archeozoology lithic knapping hafting balistics use-wear MSA Cell culture Automated concentration of viral particles in wastewater samples using Nanotrap Microbiome A Beads Nanotrap automated concentration wastewater concentration SARS-CoV-2 查看全部 Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research cell banking hiPSC master cell bank stem cell quality Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Simple, Inexpensive RNA Isolation and One-Step RT-qPCR Methods for SARS-CoV-2 Detection and General Use direct RT-qPCR M-MLV reverse transcriptase purification one-step RT-qPCR master mix SARS-CoV-2 testing Taq polymerase purification Experimental Models of Alcohol Use Disorder and Their Application for Pathophysiological Investigations alcohol use disorder alcohol vapor chamber animal behavioral paradigms two-bottle choice procedure Data Harmonization Guidelines to Combine Multi-platform Genomic Data from Admixed Populations and Boost Power in Genome-Wide Association Studies ancestry inference batch effects data harmonization genomic data merging Exploring the Genomic Landscape of Cancer Patient Cohorts with GenVisR Bioconductor R genome visualization Searching and Navigating UniProt Databases database navigation protein search tutorial UniProt Electron Microscopy of Cells Grown on Polyacrylamide Hydrogels cell culture electron microscopy Epon embedding hydrogels polyacrylamide Leaf Wax Lipid Extraction for Archaeological Applications archaeology human evolution isotope analysis lipid biomarkers paleoecology Using Mothur to Determine Bacterial Community Composition and Structure in 16S Ribosomal RNA Datasets 16S rRNA microbiome OTUs 查看全部