Generation of insulin-producing pancreatic β cells from multiple human stem cell lines

Nathaniel J. Hogrebe, Kristina G. Maxwell, Punn Augsornworawat, Jeffrey R. Millman

Published: 2021-08-03 DOI: 10.1038/s41596-021-00560-y

pluripotent stem cells

Pancreatic β cells

Stem cell differentiation

Insulin secretion

Planar culture

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We detail a six-stage planar differentiation methodology for generating human pluripotent stem cell–nobreakderived pancreatic βcells (SC-β cells) that secrete high amounts of insulin in response to glucose stimulation. This protoc
Introduction
Materials
Procedure
Troubleshooting
Timing
- Production of SC-β cells
- In vitro assays to assess SC-βnobreak-nobreakcell quality and function
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Generation of insulin-producing pancreatic β cells from multiple human stem cell lines

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