Fly Model Protocols Mel Feany Published: 2022-11-22 DOI: 10.17504/protocols.io.q26g7ypeqgwz/v1 ASAPCRN AI 解读 浏览 1064 Abstract A collection of protocols describing experimental work with fly models. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols An MSstats workflow for detecting differentially abundant proteins in large-scale data-independent acquisition mass spectrometry experiments with FragPipe processing High-throughput cultivation and identification of bacteria from the plant root microbiota A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys CODEX multiplexed tissue imaging with DNA-conjugated antibodies Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids Raman spectral cytopathology for cancer diagnostic applications Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases The use of methanol as a C1 building block 查看全部 BIDMC TMC - SOP for collection of peripheral lymphatic vessels Head-fixed behavior ASAPCRN Donor Selection Criteria for Adipose Procurement -- University of Minnesota Human TMC Adipose Human SenNet criteria UMN UMinnesota University of Minnesota Niedernhofer Donor criteria Brain Tissue Samples and Mitochondrial extracts Samples Western Blot Gel Electrophoresis Gel Gel Electrophoresis Examples of protocols on protocols.io from different disciplines Analysis of De Novo Synthesized Proteins Cell-free protein synthesis G protein-coupled receptor Protein modification Non-canonical amino acids Amber suppression Confocal laser scanning microscopy Wheat disease symptoms observation, capture, description, and evaluation Pharmacological treatment with corticoid or hyaluronic acid injections into subtalar joint via lateral access Osteoarthritis; Subtalar joint; Intra-articular injection; Hyaluronic acid; Corticoid. Immunoprecipitation from transfected cells 查看全部 Mouse and Rat Anesthesia and Analgesia analgesia anesthesia mouse rat surgery Bacterial Pathogenesis: Assessment of Intracellular Positioning of Pathogen-Containing Vacuoles During Infection bacterial pathogenesis host-pathogen interactions intracellular pathogen intracellular trafficking Salmonella enterica Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library DARPin designed ankyrin repeat protein DNA library construction next-generation sequencing protein binder protein display Preparation of a Convertible Spacer Containing a Disulfide Group for Versatile Functionalization of Oligonucleotides oligonucleotide functionalization oligonucleotide conjugation disulfide linker redox-responsive release Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification Small Interfering RNA Delivery Into Primary Human Natural Killer Cells for Functional Gene Analyses cell therapy gene function knockdown NK cells siRNA Exploring the Genomic Landscape of Cancer Patient Cohorts with GenVisR Bioconductor R genome visualization Multiplex Quantification of Plasma Biomarkers for Patients with Gaucher Disease Type 1 biomarker Gaucher disease Lyso-Gb1 Lyso-Gb1 analogs mass spectrometry N-palmitoyl-O-phosphocholineserine sphingosylphosphorylcholine Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior activity-controlled optogenetics cell-type specificity frequency specificity stimulation pattern temporal precision Generation of Tumor Spheroids to Evaluate T Cell and NK Cell Cytotoxicity 3D culture system antitumor lymphocytes cell imaging natural killer cells γδ T cells 查看全部
