Ea(52-68) uptake assays
Rebecca Wallings
Published: 2023-12-15 DOI: 10.17504/protocols.io.14egn3r3pl5d/v1
Abstract
Ea(52-68) uptake assays on pMacs
Steps
1.
Reconstitute MHC-II Ea chain (Ea) (52–68) peptide (Anaspec) in sterile distilled H2O to a final concentrate of 1mg/mL.
Once peritoneal macrophages are adhered to plates, add 5µg per well of 24-well plate and incubate for 18-hours
Harvest cells as per flow cytometry protocol and stain with eBioY-Ae antibody (ThermoFisher) to assess Ea52-68 peptide bound to I-Ab
