Deepwell Reusual Protocol

Cover a deep well with two layers of aluminium foil and tape the front and back to hold it securely. The tape also turns the aluminium foil into a lid for the deep well by temporarily peeling off the front tape.

Autoclave at 120C for 20min. After autoclaving, keep in a heated oven (> 50C) overnight for drying and desiccation.

Do not fill the deep wells more than 60% of their max volume if they are to be placed in a shaking incubator to avoid spillage.

Tape around the deep well over the aluminium foil to prevent spillage.

While carrying the deep well outside the lab space, enclose it in a waterproof autoclave bag. Keep sufficient absorbent paper on hand in case of accidental spillage.

In the autoclave facility, autoclave the deep wells at 120C for >30min to decontaminate.

Once autoclaved, the deep wells will be brought back to the lab

Carefully peel the aluminium foil and dispose the cultures and aluminium foil into a disposal bag containing paper roll to absorb the liquid.

Send decontaminated culture for incineration (yellow bin route).

Rinse the deep well plate with water and a brush to clean before future use.

Keep in a heated oven (> 50C) overnight for drying and desiccation