Cell specificity Xianjun Dong Published: 2023-11-18 DOI: 10.17504/protocols.io.6qpvr3243vmk/v1 ASAPCRN AI 解读 浏览 1065 Abstract This protocol describes the method to perform cell specificity analysis. Attachments protocol.cellspecificity.pdf Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols 3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling Dual detection of chromatin accessibility and DNA methylation using ATAC-Me Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development Probing low-copy-number proteins in single living cells using single-cell plasmonic immunosandwich assays Generation of ‘semi-guided’ cortical organoids with complex neural oscillations Comprehensive synthesis of Ti3C2Txfrom MAX phase to MXene Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2) Labeling and tracking of immune cells in ex vivo human skin 查看全部 BIDMC TMC - SOP for collection of peripheral lymphatic vessels Head-fixed behavior ASAPCRN Donor Selection Criteria for Adipose Procurement -- University of Minnesota Human TMC Adipose Human SenNet criteria UMN UMinnesota University of Minnesota Niedernhofer Donor criteria Brain Tissue Samples and Mitochondrial extracts Samples Western Blot Gel Electrophoresis Gel Gel Electrophoresis Examples of protocols on protocols.io from different disciplines Analysis of De Novo Synthesized Proteins Cell-free protein synthesis G protein-coupled receptor Protein modification Non-canonical amino acids Amber suppression Confocal laser scanning microscopy Wheat disease symptoms observation, capture, description, and evaluation Pharmacological treatment with corticoid or hyaluronic acid injections into subtalar joint via lateral access Osteoarthritis; Subtalar joint; Intra-articular injection; Hyaluronic acid; Corticoid. Immunoprecipitation from transfected cells 查看全部 Mouse and Rat Anesthesia and Analgesia analgesia anesthesia mouse rat surgery Bacterial Pathogenesis: Assessment of Intracellular Positioning of Pathogen-Containing Vacuoles During Infection bacterial pathogenesis host-pathogen interactions intracellular pathogen intracellular trafficking Salmonella enterica Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library DARPin designed ankyrin repeat protein DNA library construction next-generation sequencing protein binder protein display Preparation of a Convertible Spacer Containing a Disulfide Group for Versatile Functionalization of Oligonucleotides oligonucleotide functionalization oligonucleotide conjugation disulfide linker redox-responsive release Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification Small Interfering RNA Delivery Into Primary Human Natural Killer Cells for Functional Gene Analyses cell therapy gene function knockdown NK cells siRNA Exploring the Genomic Landscape of Cancer Patient Cohorts with GenVisR Bioconductor R genome visualization Multiplex Quantification of Plasma Biomarkers for Patients with Gaucher Disease Type 1 biomarker Gaucher disease Lyso-Gb1 Lyso-Gb1 analogs mass spectrometry N-palmitoyl-O-phosphocholineserine sphingosylphosphorylcholine Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior activity-controlled optogenetics cell-type specificity frequency specificity stimulation pattern temporal precision Generation of Tumor Spheroids to Evaluate T Cell and NK Cell Cytotoxicity 3D culture system antitumor lymphocytes cell imaging natural killer cells γδ T cells 查看全部
