An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples

Roeltje R. Maas, Klara Soukup, Florian Klemm, Mara Kornete, Robert L. Bowman, Romain Bedel, Damien N. Marie, Ángel F. Álvarez-Prado, Danny Labes, Anne Wilson, Jean-Philippe Brouland, Roy T. Daniel, Monika E. Hegi, Johanna A. Joyce

Published: 2021-08-29 DOI: 10.1038/s41596-021-00594-2

tissue processing

RNA sequencing

brain tumor

immune microenvironment

cell populations

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Human tissue samples represent an invaluable source of information for the analysis of disease-specific cellular alterations and their variation between different pathologies. In cancer research, advancing a comprehensive understanding of the unique chara
Introduction
Materials
Procedure
Troubleshooting
Timing
- Steps that need to be performed on the day of tissue reception, as soon as possible post-resection
- Steps that can be performed at a later time point
Anticipated results
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples

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