An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractablePseudomonas aeruginosaisolates

Debbie Pankratz, Nicolas Oswaldo Gomez, Agnes Nielsen, Ayten Mustafayeva, Melisa Gür, Fabián Arce-Rodriguez, Pablo Ivan Nikel, Susanne Häussler, Alejandro Arce-Rodriguez

Published: 2023-10-04 DOI: 10.1038/s41596-023-00882-z

CRISPR-Cas9

Pseudomonas aeruginosa

recombineering

genetic engineering

Ssr recombinase

AI 解读

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Stable isotope tracing to assess tumor metabolism in vivo

Measuring DNA modifications with the comet assay: a compendium of protocols

Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors

A systems-level mass spectrometry-based technique for accurate and sensitive quantification of the RNA cap epitranscriptome

Spooling electrochemiluminescence spectroscopy: development, applications and beyond

Chronic, cortex-wide imaging of specific cell populations during behavior

Chromatin accessibility profiling by ATAC-seq

Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture

Pooled genetic perturbation screens with image-based phenotypes

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References

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An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractablePseudomonas aeruginosaisolates

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