Surgical implantation of wireless, battery-free optoelectronic epidural implants for optogenetic manipulation of spinal cord circuits in mice

Jose G. Grajales-Reyes, Bryan A. Copits, Ferrona Lie, Yongjoon Yu, Raudel Avila, Sherri K. Vogt, Yonggang Huang, Anthony R. Banks, John A. Rogers, Robert W. Gereau, Judith P. Golden

Published: 2021-05-23 DOI: 10.1038/s41596-021-00532-2

Optogenetics

Wireless Optoelectronic Implants

Spinal Cord Circuits

Epidural Space

Neural Circuitry

AI 解读

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

查看全部

Sections

Figures

References

The use of optogenetics to regulate neuronal activity has revolutionized the study of the neural circuitry underlying a number of complex behaviors in rodents. Advances have been particularly evident in the study of brain circuitry and related behaviors,
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

Boyden, E. S. A history of optogenetics: the development of tools for controlling brain circuits with light. F1000 Biol. Rep. 3, 11 (2011).
Carr, F. B. & Zachariou, V. Nociception and pain: lessons from optogenetics. Front. Behav. Neurosci. 8, 69 (2014).
Samineni, V. K. et al. Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics. Pain 158, 2108–2116 (2017).
Park, S. I. S. Il et al. Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics. Nat. Biotechnol. 33, 1280–1286 (2015).
Zhang, Y. Y. et al. Battery-free, fully implantable optofluidic cuff system for wireless optogenetic and pharmacological neuromodulation of peripheral nerves. Sci. Adv. 5, 1–12 (2019).
Vázquez-Guardado, A., Yang, Y., Bandodkar, A. J. & Rogers, J. A. Recent advances in neurotechnologies with broad potential for neuroscience research. Nat. Neurosci. 23, 1522–1536 (2020).
Bridges, A. W. & García, A. J. Anti-inflammatory polymeric coatings for implantable biomaterials and devices. J. Diabetes Sci. Technol. 2, 984–994 (2008).
Bedi, S. S. et al. Chronic spontaneous activity generated in the somata of primary nociceptors is associated with pain-related behavior after spinal cord injury. J. Neurosci. 30, 14870–14882 (2010).
Djouhri, L., Koutsikou, S., Fang, X., McMullan, S. & Lawson, S. N. Spontaneous pain, both neuropathic and inflammatory, is related to frequency of spontaneous firing in intact C-fiber nociceptors. J. Neurosci. 26, 1281–1292 (2006).
DeBerry, J. J. et al. Differential regulation of bladder pain and voiding function by sensory afferent populations revealed by selective optogenetic activation. Front. Integr. Neurosci. 12, 1–13 (2018).
Montgomery, K. L. et al. Wirelessly powered, fully internal optogenetics for brain, spinal and peripheral circuits in mice. Nat. Methods 12, 969–974 (2015).
Iyer, S. M. et al. Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nat. Biotechnol. 32, 274–278 (2014).
Harding, E. K., Fung, S. W. & Bonin, R. P. Insights into spinal dorsal horn circuit function and dysfunction using optical approaches. Front. Neural Circuits 14, 1–21 (2020).
Copits, B. A., Pullen, M. Y. & Gereau, R. W. Spotlight on pain: optogenetic approaches for interrogating somatosensory circuits. Pain 157, 2424–2433 (2016).
Francois, A. et al. A brainstem-spinal cord inhibitory circuit for mechanical pain modulation by GABA and enkephalins. Neuron 93, 822–839 (2017).
Christensen, A. J. et al. In vivo interrogation of spinal mechanosensory circuits. Cell Rep 17, 1699–1710 (2016).
Lu, C. et al. Flexible and stretchable nanowire-coated fibers for optoelectronic probing of spinal cord circuits. Sci. Adv. https://doi.org/10.1126/sciadv.1600955 (2017).
Lu, L. et al. Wireless optoelectronic photometers for monitoring neuronal dynamics in the deep brain. Proc. Natl Acad. Sci. USA 115, E1374–E1383 (2018).
Wang, Y. et al. Flexible and fully implantable upconversion device for wireless optogenetic stimulation of the spinal cord in behaving animals. Nanoscale 12, 2406–2414 (2020).
Butler, R. K. & Finn, D. P. Stress-induced analgesia. Prog. Neurobiol. 88, 184–202 (2009).
Mickle, A. D. & Gereau, R. W. 4th A bright future? Optogenetics in the periphery for pain research and therapy. Pain 159, 65–73 (2018).
Madisen, L. et al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nat. Neurosci. 15, 793–802 (2012).
Bonin, R. P. et al. Epidural optogenetics for controlled analgesia. Mol. Pain 12, 1–11 (2016).
Mishra, S. K., Tisel, S. M., Orestes, P., Bhangoo, S. K. & Hoon, M. A. TRPV1-lineage neurons are required for thermal sensation. EMBO J 30, 582–593 (2011).
Cavanaugh, D. J. et al. Restriction of transient receptor potential vanilloid-1 to the peptidergic subset of primary afferent neurons follows its developmental downregulation in nonpeptidergic neurons. J. Neurosci. 31, 10119–10127 (2011).
Chaplan, S. R., Bach, F. W., Pogrel, J. W., Chung, J. M. & Yaksh, T. L. Quantitative assessment of tactile allodynia in the rat paw. J. Neurosci. Methods 53, 55–63 (1994).
Brenner, D. S., Golden, J. P. & Gereau, R. W. IV A novel behavioral assay for measuring cold sensation in mice. PLoS ONE https://doi.org/10.1371/journal.pone.0039765 (2012).
Beaudry, H., Daou, I., Ase, A. R., Ribeiro-Da-Silva, A. & Séguela, P. Distinct behavioral responses evoked by selective optogenetic stimulation of the major TRPV1+ and MrgD+ subsets of C-fibers. Pain 158, 2329–2339 (2017).
Rogers, J. A. et al. Injectable and injectable cellular-scale electronic devices. US patent 5861366 (2020).

Surgical implantation of wireless, battery-free optoelectronic epidural implants for optogenetic manipulation of spinal cord circuits in mice

Supplementary information

推荐阅读