Quantitative determination of phosphatidylethanol in dried blood spots for monitoring alcohol abstinence Marc Luginbühl, Frederike Stöth, Alexandra Schröck, Stefan Gaugler, Wolfgang Weinmann Published: 2020-12-06 DOI: 10.1038/s41596-020-00416-x AI 解读 浏览 1664 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration Deepometry, a framework for applying supervised and weakly supervised deep learning to imaging cytometry Reprogramming fibroblast into human iBlastoids Modified Neuropixels probes for recording human neurophysiology in the operating room Purification of mammalian telomeric DNA for single-molecule analysis Selective footprinting of 40S and 80S ribosome subpopulations (Sel-TCP-seq) to study translation and its control Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems MOF-derived nanoporous carbons with diverse tunable nanoarchitectures 查看全部 SOP for Immune cells isolation mouse adult brain with CD45 beads ASAPCRN Test for admin publication GRAB sensor imaging in mouse striatal slices GRAB sensors imaging mouse ex vivo slices ASAPCRN Cost-effective Approach for Assessing Chemosensory Response in Parhyale hawaiensis: A Time-to-Event Experiment Chemosensation Feeding behaviour Parhyale hawaiensis Y-Maze Time-to-event Kaplan-Meier OR Cox regression survival analysis A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection laser capture microdissection RNA extraction human islets Qubit4 Fluorometer Protocol: Invitrogen dsDNA HS Assay Kit (REF 32854) Mitochondrial isolation protocol Mitochondrial isolation HeLa cells ASAPCRN Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765) RCA-NGS for RNA viruses Oxford Nanopore Technology RNA virus Sequence method MinION Nanopore sequencing RCA-NGS DNA Extraction from 0.22µm Sterivex Filters - Qiagen Blood and Tissue with Inhibitor Removal 查看全部 Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants ADP ATP NAD+/NADP+ NADH/NADPH Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells antibody beginner clearing expansion microscopy fluorescence microscopy HeLa cells hydrogel imaging immunocytochemistry immunohistochemistry microscopy microtubules super-resolution microscopy A Platform for Medium-Throughput Cell-Free Analyses of Microtubule-Interacting Proteins Using Mammalian Cell Lysates cytoskeleton MAPs microtubules microtubule-associated proteins purification-free TIRF assays Live Imaging and Quantification of Neutrophil Extracellular Trap Formation automated quantification live imaging neutrophil extracellular trap Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis Assessing Cell Competition in Human Pluripotent Stem Cell (hPSC) Cultures cell competition culture-acquired genetic variants fitness-sensing assays fluorescent hPSC subline human pluripotent stem cells (hPSCs) single-cell cloning hPSCs Reprogramming Urine-Derived Cells Using Commercially Available Self-Replicative RNA and a Single Electroporation induced pluripotent stem cell reprogramming self-replicative RNA urine Animal Models for Toxoplasma gondii Infection cellular immunity encephalitis humoral immunity parasite retinitis A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions fungal genomics maize plant genomics plant-microbe interactions unique molecular identifiers Ustilago maydis virulence factors 查看全部