Quantitative determination of phosphatidylethanol in dried blood spots for monitoring alcohol abstinence Marc Luginbühl, Frederike Stöth, Alexandra Schröck, Stefan Gaugler, Wolfgang Weinmann Published: 2020-12-06 DOI: 10.1038/s41596-020-00416-x AI 解读 浏览 1977 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail Profiling native pulmonary basement membrane stiffness using atomic force microscopy Genetic circuit design automation with Cello 2.0 Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2) A practical guide to scanning light-field microscopy with digital adaptive optics Effective high-throughput isolation of fully human antibodies targeting infectious pathogens Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments MOF-derived nanoporous carbons with diverse tunable nanoarchitectures Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease 查看全部 Basketball Exercise basketball PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX) QUINT Workflow for Fluorescence ASAPCRN The Treadmill-Based Tripping Perturbation Fall Trip Stumble Perturbation Disturbance Cell treatments for time-lapse experiments Methodology for Frontier Model of the Environmental Inefficiency effects on Livestock Bioeconomy SFA Bieoconomic model Efficiency Western Blot A protocol for tissue clearing and three-dimensional imaging of human sigmoid mucosal biopsies Tissue clearing 3D imaging human sigmoid mucosal biopsy nerve fibers SH-SY5Y Transduced with HLA-A2 mCherry Lentivirus Sorting Protocol ASAPCRN Protocol for single cell and single nucleus prep for Cell Pellets/Multiple Myeloma (snRNA & snATAC) 查看全部 Optimized and Scalable Precoating-Free Reprogramming of Human Peripheral Blood Mononuclear Cells into iPSCs coating extracellular matrix induced pluripotent stem cells laminin-511 reprogramming PBMCs scaling down Methods to Detect Small Molecule Inhibition of RING E3 Ligase Activity alpha screen RING E3 ligase small molecules TRAF6 Ubc13 ubiquitination Reprogramming Urine-Derived Cells Using Commercially Available Self-Replicative RNA and a Single Electroporation induced pluripotent stem cell reprogramming self-replicative RNA urine Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Production and Thermal Exchange of Conditional Peptide-MHC I Multimers CD8+ T cell staining MHC class I multimer peptide thermal exchange A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein Sperm Chromatin Structure Assay (SCSA®) for Fertility Assessment DNA integrity flow cytometry male fertility pregnancy outcomes SCSA sperm chromatin structure assay ntLink: A Toolkit for De Novo Genome Assembly Scaffolding and Mapping Using Long Reads de novo genome assembly long reads minimizer mapping scaffolding ntLink An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat Agrobacterium CRISPR genome editing immature embryo wheat transformation 查看全部
