Protocols and risks: when less is more Jaspreet Pannu, Jonas B. Sandbrink, Matthew Watson, Megan J. Palmer, David A. Relman Published: 2021-12-05 DOI: 10.1038/s41596-021-00655-6 AI 解读 浏览 1168 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Inferring cellular and molecular processes in single-cell data with non-negative matrix factorization using Python, R and GenePattern Notebook implementations of CoGAPS Dual detection of chromatin accessibility and DNA methylation using ATAC-Me Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView Stabilization and structure determination of integral membrane proteins by termini restraining Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds Design, assembly, and characterization of membrane-spanning DNA nanopores Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance Analysis framework and experimental design for evaluating synergy-driving gene expression Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay 查看全部 Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 CHOP TMC Single Cell Multiome ATAC + Gene Expression HuBMAP Children's Hospital of Philadelphia TMC Cloning individual AAV capsid variants ASAPCRN Cell culture Targeted ExSeq -- In Situ Sequencing (Illumina Chemistry) expansion microscopy in situ sequencing expansion sequencing targeted ExSeq ExSeq spatial transcriptomics spatial omics spatially resolved transcriptomics Well-tempered Metadynamics protocol Synaptic immunohistochemistry - wholemount via acetone permeabilization Recombinant αS purification Protocol ASAPCRN protocols.io academic and non-profit contract Panicle Ratio Network: A high-throughput dynamic phenotype recognition model based on ultra-high-definition unmanned aerial vehicle images for rice panicle analysis in fields 查看全部 Microglial Phagocytosis/Cell Health High-Content Assay cell health high-content analysis microglia phagocytosis quantitative phenotypic assay Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library DARPin designed ankyrin repeat protein DNA library construction next-generation sequencing protein binder protein display Cap 1 Messenger RNA Synthesis with Co-transcriptional CleanCap® Analog by In Vitro Transcription cap 1 cap analog CleanCap in vitro transcription messenger RNA Working with Worms: Caenorhabditis elegans as a Model Organism C. elegans education genetics overview A Computational Workflow for Analysis of 3′ Tag-Seq Data computational pipeline differential gene expression RNA sequencing 3′ Tag-Seq STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq RNA-Seq Data Analysis: A Practical Guide for Model and Non-Model Organisms assembly differential expression non-model plants plotting reproducibility RNA-seq In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum cell culture in vitro cultivation laboratory growth spirochetes syphilis Treponema pallidum Quantification of GTPase Cycling Rates of GTPases and GTPase:Effector Mixtures Using GTPase Glo Assays Cdc42 GTPase activity GTPase assay GTPase cycling rates GTPase effectors Using EMBL-EBI Services via Web Interface and Programmatically via Web Services bioinformatics resources bioinformatics tools programmatic access REST SOAP web services workflows 查看全部
