Precision genome editing using cytosine and adenine base editors in mammalian cells

Tony P. Huang, Gregory A. Newby, David R. Liu

Published: 2021-01-17 DOI: 10.1038/s41596-020-00450-9

single-nucleotide polymorphisms

AI 解读

Supplementary information

Supplementary Tables 1–6

Additional information on base editor components and a calculator for determining HTS library concentrations.

Supplementary Data 1

A plasmid map annotated with example primer sequences (Table 2) for cloning a base editor into a protein expression vector

Sequencing files associated with the data shown in Fig. 8, d and e. The raw fastq files are provided, along with a CRISPRessoBatch parameter file for each amplicon in the pre-processing folder. Successfully analyzed files can be found in the post-processing folder.

Supplementary Data 3

Sequencing files associated with the data shown in Fig. 8, f and g. The raw fastq files are provided, along with a CRISPRessoBatch parameter file for each amplicon. Fastq files are named by the delivery method used.

An operant social self-administration and choice model in mice

Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging

Isotropic imaging across spatial scales with axially swept light-sheet microscopy

Snapshot photoacoustic topography through an ergodic relay of optical absorption in vivo

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Algorithmic assessment of cellular senescence in experimental and clinical specimens

Creating custom synthetic genomes inEscherichia coliwith REXER and GENESIS

High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation

References

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Precision genome editing using cytosine and adenine base editors in mammalian cells

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