Mycology media

WA+C agar

Water agar + chloramphenicol (氯黴素 kill bacteria): As an environmental isolating media

A	B
Component	Amount
Agar	20g
Chloramphenicol	0.05g
DI water	1000 ml

chloramphenicol is autoclavable

PDA+C agar

potato dextrose agar + chloramphenicol (氯黴素 kill bacteria): Use when WA+C agar is not working

A	B
Component	Amount
PDA	39g (or per instruction on label)
Agar	5g
Chloramphenicol	0.05g
DI water	1000 ml

quarter-strength PDA

1. Less nutrient than regular PDA (1/4)

2. For beetle-associated fungi isolation

A	B
Component	Amount
PDA	9.75g (or per instruction on label)
Agar	5g
DI water	1000 ml

MEA (malt extraction agar)

For isolate subculture

A	B
Component	Amount
MEA	33.6g (or per instruction on label)
Agar	5g
DI water	1000 ml

Emerson YpSs Agar : For chytrids

A	B
Component	Amount
Yeast extract	4g
Soluble Starch	15g
K2HPO4	1g
MgSO4 x 7H2O	0.5g
Agar	20g
DI water	1000ml

Adujust pH to 7.0 +/- 0.2. Autoclave at 121ºC.

10% Unclarified V8 Agar

1. Combine 100 ml V8 juice and 900 ml of distilled water.
2. Add 1g CaCO₃and 0.05 g b-sitosterol and mix well. Leave stir bar in the flask for later mixing.
3. Add 15g of agar.
4. Autoclave at 15 psi for 20 minutes.
5. Stir medium while dispensing to insure good mixing of CaCO₃. 20% : 200 ml V8, 800ml d. water, 2g CaCO₃, and 0.05g b-sitosterol.

15% : 150 ml V8, 850 ml d. water, 1.5g CaCO₃, and 0.05g b-sitosterol.

Reference: Miller, P. M. 1955. V-8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 45:461-462.

SceSel+ medium (Kaltseis et al.,2009)

For isolation of Scedosporium

A	B
Component	Amount
Malt extract	6.25 g
maltose	6.25 g
KH2PO4	1.25 g
yeast extract	1 g
Magnesium Sulfate Heptahydrate	0.625 g
soy peptone	0.625 g
chloramphenicol	0.1 g
ciprofloxacin	0.1 g
streptomycin sulfate	0.1 g
dichloran	2 mg
benomyl	6 mg
DI water	1000ml