Quantification of TH immunoreactivity

TH signals from bilateral striatum and cortex are imaged under a 20x objective lens using identical imaging settings.

Open the confocal files in Image J software.

Using the “Rectangle” function to select an area (width x high = 150 x 150) in striatum sections from both hemispheres for TH quantification.

Using the “Measure” function to measure the gray value of the selected area and write down the mean value of striatum slice, M^striatum-LHS and M^striatum-RHS.

Using the “Rectangle” function to select an area (width x high = 150 x 150) in cortical sections from both hemispheres for TH quantification.

Using the “Measure” function to measure the gray value of the selected area and write down the mean value of cortical slice, M^cortex-LHSand M^cortex-RHS.

TH quantification = .