Membrane Tube Assay
Liv Jensen
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Abstract
This protocol details about the Membrane Tube Assay.
Attachments
Steps
Membrane Tube Assay
Passivate flow cell with 1mg/mL BSA in imaging buffer.
Rinse flow cell with 2 flow cell volumes of imaging buffer.
Mix GUVs with fluorescent protein and add to flow cell, allowing GUVs to settle on the bottom surface of the flow cell.
Add 1µL of a 1:1000 dilution of silica beads to flow cell.
Trap a bead in the optical trap, bring into contact with a GUV, and retract, forming a membrane tube.
Visualize protein recruitment to membrane tube with confocal microscopy.
