Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

Daesik Kim, Beum-Chang Kang, Jin-Soo Kim

Published: 2021-01-17 DOI: 10.1038/s41596-020-00453-6

Digenome-seq

off-target sites

CRISPR

nucleases

deaminases

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Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

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