Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

Daesik Kim, Beum-Chang Kang, Jin-Soo Kim

Published: 2021-01-17 DOI: 10.1038/s41596-020-00453-6

Digenome-seq

off-target sites

CRISPR

nucleases

deaminases

AI 解读

Supplementary information

Reporting Summary

3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development

Probing low-copy-number proteins in single living cells using single-cell plasmonic immunosandwich assays

Generation of ‘semi-guided’ cortical organoids with complex neural oscillations

Comprehensive synthesis of Ti3C2Txfrom MAX phase to MXene

Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

Labeling and tracking of immune cells in ex vivo human skin

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Supplementary information

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Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

Supplementary information

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