Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Haoqi Du, Hanjie Yu, Fuquan Yang, Zheng Li

Published: 2021-06-06 DOI: 10.1038/s41596-021-00544-y

High-throughput

Glycosphingolipids

Lectin microarray

MALDI-TOF mass spectrometry

Glycan analysis

AI 解读

3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development

Probing low-copy-number proteins in single living cells using single-cell plasmonic immunosandwich assays

Generation of ‘semi-guided’ cortical organoids with complex neural oscillations

Comprehensive synthesis of Ti3C2Txfrom MAX phase to MXene

Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

Labeling and tracking of immune cells in ex vivo human skin

查看全部

Sections

Figures

References

Glycosphingolipids (GSLs) are ubiquitous glycoconjugates present on the cell membrane; they play significant roles in many bioprocesses such as cell adhesion, embryonic development, signal transduction and carcinogenesis. Analyzing such amphiphilic molecu
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- MALDI-TOF/TOF MS of GSL glycans
- References
References
ACKNOWLEDGMENTS

Levery, S. B. Glycosphingolipid structural analysis and glycosphingolipidomics. Methods Enzymol. 405, 300–369 (2005).
D’Angelo, G., Capasso, S., Sticco, L. & Russo, D. Glycosphingolipids: synthesis and functions. FEBS J. 280, 6338–6353 (2013).
Dauner, M., Batroff, E., Bachmann, V., Hauck, C. R. & Wittmann, V. Synthetic glycosphingolipids for live-cell labeling. Bioconjug. Chem. 27, 1624–1637 (2016).
Platt, F. M. Sphingolipid lysosomal storage disorders. Nature 510, 68–75 (2014).
Shayman, J. A. & Larsen, S. D. The development and use of small molecule inhibitors of glycosphingolipid metabolism for lysosomal storage diseases. J. Lipid Res. 55, 1215–1225 (2014).
Ho, M. Y., Yu, A. L. & Yu, J. Glycosphingolipid dynamics in human embryonic stem cell and cancer: their characterization and biomedical implications. Glycoconj. J. 34, 765–777 (2017).
Woeste, M. A. & Wachten, D. The enigmatic role of GBA2 in controlling locomotor function. Front. Mol. Neurosci. 10, 386 (2017).
Zhao, X. et al. Lipidomic profiling links the Fanconi anemia pathway to glycosphingolipid metabolism in head and neck cancer cells. Clin. Cancer Res. 24, 2700–2709 (2018).
Fujiwara, Y., Hama, K. & Yokoyama, K. Mass spectrometry in combination with a chiral column and multichannel-MRM allows comprehensive analysis of glycosphingolipid molecular species from mouse brain. Carbohydr. Res. 490, 107959 (2020).
Ivleva, V. B. et al. Coupling thin-layer chromatography with vibrational cooling matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for the analysis of ganglioside mixtures. Anal. Chem. 76, 6484–6491 (2004).
Kyogashima, M. et al. Rapid demonstration of diversity of sulfatide molecular species from biological materials by MALDI-TOF MS. Glycobiology 16, 719–728 (2006).
Gabius, H. J., Andre, S., Jimenez-Barbero, J., Romero, A. & Solis, D. From lectin structure to functional glycomics: principles of the sugar code. Trends Biochem. Sci. 36, 298–313 (2011).
Du, H. et al. Analysis of glycosphingolipid glycans by lectin microarrays. Anal. Chem. 91, 10663–10671 (2019).
Pilobello, K. T. & Mahal, L. K. Lectin microarrays for glycoprotein analysis. Methods Mol. Biol. 385, 193–203 (2007).
Yu, H., Shu, J. & Li, Z. Lectin microarrays for glycoproteomics: an overview of their use and potential. Expert Rev. Proteomics 17, 27–39 (2020).
Hsu, K. L. & Mahal, L. K. A lectin microarray approach for the rapid analysis of bacterial glycans. Nat. Protoc. 1, 543–549 (2006).
Kuno, A. et al. Evanescent-field fluorescence-assisted lectin microarray: a new strategy for glycan profiling. Nat. Methods 2, 851–856 (2005).
Charles, P. T. et al. Fabrication and characterization of 3D hydrogel microarrays to measure antigenicity and antibody functionality for biosensor applications. Biosens. Bioelectron. 20, 753–764 (2004).
Hirabayashi, J., Yamada, M., Kuno, A. & Tateno, H. Lectin microarrays: concept, principle and applications. Chem. Soc. Rev. 42, 4443–4458 (2013).
Angeloni, S. et al. Glycoprofiling with micro-arrays of glycoconjugates and lectins. Glycobiology 15, 31–41 (2005).
Pilobello, K. T., Krishnamoorthy, L., Slawek, D. & Mahal, L. K. Development of a lectin microarray for the rapid analysis of protein glycopatterns. Chembiochem 6, 985–989 (2005).
Hsu, K. L., Pilobello, K. T. & Mahal, L. K. Analyzing the dynamic bacterial glycome with a lectin microarray approach. Nat. Chem. Biol. 2, 153–157 (2006).
Krishnamoorthy, L., Bess, J. W. Jr., Preston, A. B., Nagashima, K. & Mahal, L. K. HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin. Nat. Chem. Biol. 5, 244–250 (2009).
Hiono, T. et al. Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses. Virology 527, 132–140 (2019).
Zheng, T., Peelen, D. & Smith, L. M. Lectin arrays for profiling cell surface carbohydrate expression. J. Am. Chem. Soc. 127, 9982–9983 (2005).
Pilobello, K. T., Slawek, D. E. & Mahal, L. K. A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome. Proc. Natl Acad. Sci. USA 104, 11534–11539 (2007).
Tateno, H. et al. A novel strategy for mammalian cell surface glycome profiling using lectin microarray. Glycobiology 17, 1138–1146 (2007).
Hsu, K. L., Pilobello, K., Krishnamoorthy, L. & Mahal, L. K. Ratiometric lectin microarray analysis of the mammalian cell surface glycome. Methods Mol. Biol. 671, 117–131 (2011).
Bird-Lieberman, E. L. et al. Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett’s esophagus. Nat. Med. 18, 315–321 (2012).
Matsuda, A. et al. Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections. Biochem. Biophys. Res. Commun. 370, 259–263 (2008).
Tozawa-Ono, A. et al. Glycan profiling using formalin-fixed, paraffin-embedded tissues: Hippeastrum hybrid lectin is a sensitive biomarker for squamous cell carcinoma of the uterine cervix. J. Obstet. Gynaecol. Res. 43, 1326–1334 (2017).
Zou, X. et al. A standardized method for lectin microarray-based tissue glycome mapping. Sci. Rep. 7, 43560 (2017).
Hu, S. & Wong, D. T. Lectin microarray. Proteomics Clin. Appl. 3, 148–154 (2009).
Narimatsu, H. et al. A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics. FEBS J. 277, 95–105 (2010).
Qin, X. et al. Comparative analysis for glycopatterns and complex-type N-glycans of glycoprotein in sera from chronic hepatitis B- and C-infected patients. Front. Physiol. 8, 596 (2017).
Yamashita, K. et al. Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer. Gastric Cancer 19, 531–542 (2016).
Qin, Y. et al. Alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV. Glycoconj. J. 33, 125–136 (2016).
Takayama, H. et al. Altered glycosylation associated with dedifferentiation of hepatocellular carcinoma: a lectin microarray-based study. BMC Cancer 20, 192 (2020).
Inoue, K. et al. Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. PLoS ONE 8, e77118 (2013).
Zhu, H. et al. Glycopatterns of urinary protein as new potential diagnosis indicators for diabetic nephropathy. J. Diabetes Res. 2017, 5728087 (2017).
Qin, Y. et al. Age- and sex-associated differences in the glycopatterns of human salivary glycoproteins and their roles against influenza A virus. J. Proteome Res. 12, 2742–2754 (2013).
Shu, J. et al. Salivary glycopatterns as potential biomarkers for diagnosis of gastric cancer. Oncotarget 8, 35718–35727 (2017).
Liu, X. et al. Salivary glycopatterns as potential biomarkers for screening of early-stage breast cancer. EBioMedicine 28, 70–79 (2018).
Ebe, Y. et al. Application of lectin microarray to crude samples: differential glycan profiling of lec mutants. J. Biochem. 139, 323–327 (2006).
Hirabayashi, J. Concept, strategy and realization of lectin-based glycan profiling. J. Biochem. 144, 139–147 (2008).
Propheter, D. C., Hsu, K. L. & Mahal, L. K. Recombinant lectin microarrays for glycomic analysis. Methods Mol. Biol. 723, 67–77 (2011).
Ribeiro, J. P. & Mahal, L. K. Dot by dot: analyzing the glycome using lectin microarrays. Curr. Opin. Chem. Biol. 17, 827–831 (2013).
Hu, D., Tateno, H., Kuno, A., Yabe, R. & Hirabayashi, J. Directed evolution of lectins with sugar-binding specificity for 6-sulfo-galactose. J. Biol. Chem. 287, 20313–20320 (2012).
Fahy, E., Sud, M., Cotter, D. & Subramaniam, S. LIPID MAPS online tools for lipid research. Nucleic Acids Res. 35, W606–W612 (2007).
Rozman, M., Fabris, D., Mrla, T. & Vukelic, Z. Database and data analysis application for structural characterization of gangliosides and sulfated glycosphingolipids by negative ion mass spectrometry. Carbohydr. Res. 400, 1–8 (2014).
Huang, F. T., Han, Y. B., Feng, Y. & Yang, G. Y. A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production. J. Lipid Res. 56, 1836–1842 (2015).
Chen, W. et al. N-glycan profiles in H9N2 avian influenza viruses from chicken eggs and human embryonic lung fibroblast cells. J. Virol. Methods 249, 10–20 (2017).
Liang, Y. et al. Differentially expressed glycosylated patterns of α-1-antitrypsin as serum biomarkers for the diagnosis of lung cancer. Glycobiology 25, 331–340 (2015).
Song, X. et al. Oxidative release of natural glycans for functional glycomics. Nat. Methods 13, 528–534 (2016).
Bisel, B., Pavone, F. S. & Calamai, M. GM1 and GM2 gangliosides: recent developments. Biomol. Concepts 5, 87–93 (2014).

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Supplementary information

推荐阅读