Irreversible light-activated SpyLigation mediates split-protein assembly in 4D

Brizzia G. Munoz-Robles, Cole A. DeForest

Published: 2024-01-22 DOI: 10.1038/s41596-023-00938-0

LASL

SpyLigation

split-protein assembly

photocaging

protein activation

AI 解读

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References

Introduction
Materials
Procedure 1: Lys(oNB) synthesis
- Synthesis of 2-nitrobenzyl-N-succinimidyl carbonate
- Synthesis of Lys(oNB)
Procedure 2: split-protein design
Procedure 3: E. coli protein expression
Procedure 4: biomaterial incorporation
Procedure 5: mammalian cell culture and characterization
Troubleshooting
Timing
Anticipated results
Acknowledgements

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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D

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