Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

Federica Pinto, Nicola Segata, Coral Barcenilla, José F. Cobo-Díaz, Francesca De Filippis, Vincenzo Valentino, Raul Cabrera Rubio, Dominic O’Neil, Lisa Mahler de Sanchez, Federica Armanini, Niccolò Carlino, Aitor Blanco-Míguez, Inés Calvete-Torre, Carlos Sabater, Susana Delgado, Patricia Ruas-Madiedo, Narciso M. Quijada, Monika Dzieciol, Sigurlaug Skírnisdóttir, Stephen Knobloch, Alba Puente, Mercedes López, Miguel Prieto, Viggó Thór Marteinsson, Martin Wagner, Abelardo Margolles, Paul D. Cotter, Danilo Ercolini, Avelino Alvarez-Ordóñez

Published: 2024-01-24 DOI: 10.1038/s41596-023-00949-x

DNA extraction

microbiome analysis

food-processing environments

whole-metagenome sequencing

metagenome assembled genomes

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Supplementary information

Supplementary Information

Supplementary Fig. 1, Note and Methods

Reporting Summary

Supplementary Video 1

Microbiome mapping in the food industry: a detailed visual procedure on how to prepare the materials and take the samples at a food-processing facility. The steps that should be followed in the laboratory for sample pre-processing are shown.

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Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

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