Routine culture and study of adult human brain cells from neurosurgical specimens

Mike Dragunow, Thomas I.-H. Park, Leon C. D. Smyth, Miranda Aalderink, Zoe R. Woolf, Justin Rustenhoven, Kevin Lee, Deidre Jansson, Amy Smith, Sheryl Feng, Jason Correia, Peter Heppner, Patrick Schweder, Edward Mee

Published: 2022-01-11 DOI: 10.1038/s41596-021-00637-8

neurosurgical specimens

human brain cells

in vitro culture

cell types

neuroscience research

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When modeling disease in the laboratory, it is important to use clinically relevant models. Patient-derived human brain cells grown invitro to study and test potential treatments provide such a model. Here, we present simple, highly reproducible coordina
Introduction
Materials
Procedure 1: dissociated cultures
Procedure 2: intact tissue culture of brain organotypic slice cultures
- Before preparing organotypic cortical slice culture
- Organotypic cortical slice cultures
Procedure 3: intact tissue culture of leptomeningeal explant cultures
- Subculturing and passaging cells from explants (for low-medium cultures only)
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Routine culture and study of adult human brain cells from neurosurgical specimens

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