Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

Dylan G. Maghini, Eli L. Moss, Summer E. Vance, Ami S. Bhatt

Published: 2020-12-03 DOI: 10.1038/s41596-020-00424-x

nanopore sequencing

long-read sequencing

DNA extraction

metagenomic assembly

human gut microbiome

AI 解读

Supplementary information

Supplementary Information

Supplementary Fig. 1 and Supplementary Notes 1 and 2.

Reporting Summary

Supplementary Table 1

Summary of HMW DNA extraction methods

Supplementary Table 2

Lytic enzyme activity

Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Identification, isolation and analysis of human gut-associated lymphoid tissues

Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX

Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues

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References

Supplementary information

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Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

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