Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq Sunny Y. Yang, David Monchaud, Judy M. Y. Wong Published: 2022-02-08 DOI: 10.1038/s41596-021-00671-6 G-quadruplexes G4RP-seq RNA profiling cross-linking BioTASQ AI 解读 浏览 1694 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Metagenome-assembled genome extraction and analysis from microbiomes using KBase Selected ion flow tube mass spectrometry for targeted analysis of volatile organic compounds in human breath Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales Genetic in vivo engineering of human T lymphocytes in mouse models Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness 查看全部 Non-guided neural organoids differentiation ASAPCRN Non-Photochemical Quenching (NPQ) Relaxation Analysis by CF imager FluorCam FC800-331 and FluorCam 7 Software protocols.io academic and non-profit contract Transformation Protocol quick ligation transformation comp cells Transformation Competent cells Participant Registration Form For Mapping the Early Childhood Gut Across Ancestry, Geography, and Environment (Gut-AGE) S-2 SOIL PROCESSING soil processing QUBIT FLUOROMETER SAMPLE LYSIS EXTRACTION Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay ASAPCRN LRRK2 Rab12 Co-IP Immunoprecipitation Co-immunoprecipitation Very low-density lipoprotein receptor (VLDLR)-C-tag purification from HEK293E cells ASAPCRN Immunohistochemistry for brain sections ASAPCRN immunohistochemistry brain mouse Electrochemical analysis of laser-inscribed graphene electrodes using cyclic voltammetry (ferri/ferrocyanide redox couple) 查看全部 Optimized Workflow for Whole Genome and Transcriptome Next-Generation Sequencing of Single Cells or Limited Nucleic Acid Samples genomics MDA transcriptomics viral nucleic acid next-generation sequencing single cell A Sensitive and Quantitative mKeima Assay for Mitophagy via FACS FACS mitophagy mKeima Parkin PINK1 Let's Get Rolling: Precise Control of Microfluidic Assay Conditions to Recapitulate Selectin-Mediated Rolling Interactions of the Leukocyte Adhesion Cascade immune cells leukocyte adhesion cascade microfluidics neutrophil rolling selectin Massively Parallel Sequencing of the Mitogenome from Human Hair Shafts in Forensic Investigations DNA extraction of human hair forensic genetics mitochondrial genome MPS multiplex PCR Measuring Changes in Keap1-Nrf2 Protein Complex Conformation in Individual Cells by FLIM-FRET image analysis Keap1 Nrf2 time-lapse FLIM Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 SARS-CoV-2 COVID-19 COVID19 virus quantification plaque assay Tissue-Specific Isolation of Tagged Arabidopsis Plastids Arabidopsis biotin-streptavidin interaction editable Golden Gate plasmids plastids tissue-specific isolation A Low-Cost Tebuconazole-Based Screening Test for Azole-Resistant Aspergillus fumigatus antifungal Aspergillus fumigatus azole resistance fungal diagnostic tebuconazole Chromosome Analysis and Sorting Using Conventional Flow Cytometers chromosomes DAPI flow karyotyping propidium iodide Characterization of Klebsiella pneumoniae Extracellular Polysaccharides capsule hypermucoviscosity extracellular polysaccharide Klebsiella pneumoniae silver stain 查看全部