Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

Malosree Maitra, Corina Nagy, Anjali Chawla, Yu Chang Wang, Camila Nascimento, Matthew Suderman, Jean-François Théroux, Naguib Mechawar, Jiannis Ragoussis, Gustavo Turecki

Published: 2021-05-09 DOI: 10.1038/s41596-021-00514-4

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Reporting Summary

Preparation of single-cell suspensions of mouse glomeruli for high-throughput analysis

cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements)

Genome-wide pooled CRISPR screening in neurospheres

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness

Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells

Scanorama: integrating large and diverse single-cell transcriptomic datasets

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Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

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