Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

Hiroyuki Koike, Kentaro Iwasawa, Rie Ouchi, Mari Maezawa, Masaki Kimura, Asuka Kodaka, Shozo Nishii, Wendy L. Thompson, Takanori Takebe

Published: 2021-01-10 DOI: 10.1038/s41596-020-00441-w

organoids

pluripotent stem cells

hepato-biliary-pancreatic development

inter-organ connectivity

3D culture

AI 解读

Supplementary information

Reporting Summary

Supplementary Video 1

Animated overview of the HBPO protocol

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Supplementary information

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Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

Supplementary information

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