Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq

J. Keith Joung, Martin J. Aryee, Nikolay L. Malinin, GaHyun Lee, Cicera R. Lazzarotto, Yichao Li, Zongli Zheng, Nhu T. Nguyen, Matthew Liebers, Ved V. Topkar, A. John Iafrate, Long P. Le, Shengdar Q. Tsai

Published: 2021-11-11 DOI: 10.1038/s41596-021-00626-x

off-target sites

high-throughput sequencing

GUIDE-seq

genome-editing

nuclease activity

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Supplementary information

Reporting Summary

Supplementary Data

Example Illumina SampleSheet SampleSheet for NGS sequencing of GUIDE-seq samples. Demultiplexing is built into the pipeline and library’s indices shouldn’t be specified here. All sequence reads will be sorted into an Undetermined category used for further processing. However, it is important to add an extra eight As after index 2 to include reading of UMI required by the pipeline.

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The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

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Profiling native pulmonary basement membrane stiffness using atomic force microscopy

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Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

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Supplementary information

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Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq

Supplementary information

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