A surgical mouse model of neonatal pressure overload by transverse aortic constriction

Mona Malek Mohammadi, Aya Abouissa, Joerg Heineke

Published: 2020-12-15 DOI: 10.1038/s41596-020-00434-9

neonatal mouse model

pressure overload

transverse aortic constriction

cardiac regeneration

therapeutic strategies

AI 解读

Supplementary information

Supplementary Information

Supplementary Materials and Methods.

Reporting Summary

Supplementary Video 1

Video demonstrating the nTAC procedure

Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Identification, isolation and analysis of human gut-associated lymphoid tissues

Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX

Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices

A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues

查看全部

Sections

Figures

References

Supplementary information

Hein, S. et al. Progression from compensated hypertrophy to failure in the pressure-overloaded human heart: structural deterioration and compensatory mechanisms. Circulation 107, 984–991 (2003).
Zhou, P. & Pu, W. T. Recounting cardiac cellular composition. Circ. Res. 118, 368–370 (2016).
Hesse, M., Welz, A. & Fleischmann, B. K. Heart regeneration and the cardiomyocyte cell cycle. Pflugers Arch. 470, 241–248 (2018).
Alkass, K. et al. No evidence for cardiomyocyte number expansion in preadolescent mice. Cell 163, 1026–1036 (2015).
Walsh, S., Ponten, A., Fleischmann, B. K. & Jovinge, S. Cardiomyocyte cell cycle control and growth estimation in vivo—an analysis based on cardiomyocyte nuclei. Cardiovasc. Res. 86, 365–373 (2010).
Maillet, M., van Berlo, J. H. & Molkentin, J. D. Molecular basis of physiological heart growth: fundamental concepts and new players. Nat. Rev. Mol. Cell Biol. 14, 38–48 (2013).
Heineke, J. & Molkentin, J. D. Regulation of cardiac hypertrophy by intracellular signalling pathways. Nat. Rev. Mol. Cell Biol. 7, 589–600 (2006).
Grund, A. & Heineke, J. Exercise makes the difference: deconstructing physiological hypertrophy in swine. J. Mol. Cell Cardiol. 79, 89–91 (2015).
Shimizu, I. & Minamino, T. Physiological and pathological cardiac hypertrophy. J. Mol. Cell. Cardiol. 97, 245–262 (2016).
Nakamura, M. & Sadoshima, J. Mechanisms of physiological and pathological cardiac hypertrophy. Nat. Rev. Cardiol. 15, 387–407 (2018).
Porrello, E. R. et al. Transient regenerative potential of the neonatal mouse heart. Science 331, 1078–1080 (2011).
Malek Mohammadi, M. et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol. Med. 9, 265–279 (2017).
Zhu, W. et al. Regenerative potential of neonatal porcine hearts. Circulation 138, 2809–2816 (2018).
Ye, L. et al. Early regenerative capacity in the porcine heart. Circulation 138, 2798–2808 (2018).
Vujic, A. et al. Exercise induces new cardiomyocyte generation in the adult mammalian heart. Nat. Commun. 9, 1659 (2018).
Perrino, C. et al. Intermittent pressure overload triggers hypertrophy-independent cardiac dysfunction and vascular rarefaction. J. Clin. Invest. 116, 1547–1560 (2006).
Malek Mohammadi, M. et al. Induction of cardiomyocyte proliferation and angiogenesis protects neonatal mice from pressure overload-associated maladaptation. JCI Insight https://doi.org/10.1172/jci.insight.128336 (2019).
Rockman, H. A. et al. Segregation of atrial-specific and inducible expression of an atrial natriuretic factor transgene in an in vivo murine model of cardiac hypertrophy. Proc. Natl Acad. Sci. USA 88, 8277–8281 (1991).
Patten, R. D. & Hall-Porter, M. R. Small animal models of heart failure: development of novel therapies, past and present. Circ. Heart Fail. 2, 138–144 (2009).
Heineke, J. Screening for novel calcium-binding proteins that regulate cardiac hypertrophy: CIB1 as an example. Methods Mol. Biol. 963, 279–301 (2013).
Hartley, C. J. et al. Doppler velocity measurements from large and small arteries of mice. Am. J. Physiol. Heart Circ. Physiol. 301, H269–H278 (2011).
Tavakoli, R., Nemska, S., Jamshidi, P., Gassmann, M. & Frossard, N. Technique of minimally invasive transverse aortic constriction in mice for induction of left ventricular hypertrophy. J. Vis. Exp. https://doi.org/10.3791/56231 (2017).
Aurora, A. B. et al. Macrophages are required for neonatal heart regeneration. J. Clin. Invest. 124, 1382–1392 (2014).
Mahmoud, A. I. et al. Nerves regulate cardiomyocyte proliferation and heart regeneration. Dev. Cell 34, 387–399 (2015).
Karra, R. & Poss, K. D. Redirecting cardiac growth mechanisms for therapeutic regeneration. J. Clin. Invest. 127, 427–436 (2017).
Cui, M., Wang, Z., Bassel-Duby, R. & Olson, E. N. Genetic and epigenetic regulation of cardiomyocytes in development, regeneration and disease. Development https://doi.org/10.1242/dev.171983 (2018).
O’Tierney, P. F. et al. Reduced systolic pressure load decreases cell-cycle activity in the fetal sheep heart. Am. J. Physiol. Regul. Integ.r Comp. Physiol. 299, R573–R578 (2010).
Canseco, D. C. et al. Human ventricular unloading induces cardiomyocyte proliferation. J. Am. Coll. Cardiol. 65, 892–900 (2015).
Wohlschlaeger, J. et al. Hemodynamic support by left ventricular assist devices reduces cardiomyocyte DNA content in the failing human heart. Circulation 121, 989–996 (2010).
Mahmoud, A. I., Porrello, E. R., Kimura, W., Olson, E. N. & Sadek, H. A. Surgical models for cardiac regeneration in neonatal mice. Nat. Protoc. 9, 305–311 (2014).
Polizzotti, B. D., Ganapathy, B., Haubner, B. J., Penninger, J. M. & Kuhn, B. A cryoinjury model in neonatal mice for cardiac translational and regeneration research. Nat. Protoc. 11, 542–552 (2016).
deAlmeida, A. C., van Oort, R. J. & Wehrens, X. H. Transverse aortic constriction in mice. J. Vis. Exp. https://doi.org/10.3791/1729 (2010).
Wang, Z. et al. Mechanistic basis of neonatal heart regeneration revealed by transcriptome and histone modification profiling. Proc. Natl Acad. Sci. USA 116, 18455–18465 (2019).
Li, J. et al. Regulatory T-cells are required for neonatal heart regeneration. Preprint at bioRxiv https://doi.org/10.1101/355065 (2018).
Samak, M. et al. Cardiac hypertrophy: an introduction to molecular and cellular basis. Med. Sci. Monit. Basic Res. 22, 75–79 (2016).
Kilkenny, C., Browne, W. J., Cuthill, I. C., Emerson, M. & Altman, D. G. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol 8, e1000412 (2010).
Appari, M. et al. C1q-TNF-related protein-9 promotes cardiac hypertrophy and failure. Circ. Res. 120, 66–77 (2017).
Grund, A. et al. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation. EMBO Mol. Med. 11, e10018 (2019).
Heineke, J. et al. CIB1 is a regulator of pathological cardiac hypertrophy. Nat. Med. 16, 872–879 (2010).
Haubner, B. J., Schuetz, T. & Penninger, J. M. A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice. Basic Res. Cardiol. 111, 64 (2016).
Hesse, M. et al. Midbody positioning and distance between daughter nuclei enable unequivocal identification of cardiomyocyte cell division in mice. Circ. Res. 123, 1039–1052 (2018).
Li, Y. H. et al. Effect of age on peripheral vascular response to transverse aortic banding in mice. J. Gerontol. A 58, B895–B899 (2003).

A surgical mouse model of neonatal pressure overload by transverse aortic constriction

Supplementary information

推荐阅读