A chemo-mechanical cochleostomy preserves hearing for the in vivo functional imaging of cochlear cells

Jinkyung Kim, Anthony J. Ricci

Published: 2023-01-03 DOI: 10.1038/s41596-022-00786-4

in vivo imaging

cochlear cells

hearing preservation

chemo-mechanical cochleostomy

otic capsule bone

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Supplementary information

Reporting Summary

Supplementary Video 1

Imaging window creation

Supplementary Data 1

Mouse head holder, part1.

Supplementary Data 2

Mouse head holder, part2.

Supplementary Data 3

Mouse head holder, part3.

Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization

Hi-CO: 3D genome structure analysis with nucleosome resolution

Practical and concise synthesis of nucleoside analogs

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools

Capture of the newly transcribed RNA interactome using click chemistry

Design, assembly, and characterization of membrane-spanning DNA nanopores

Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq

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Yin, L. et al. Imaging light responses of retinal ganglion cells in the living mouse eye. J. Neurophysiol. 109, 2415–2421 (2013).
Charpak, S., Mertz, J., Beaurepaire, E., Moreaux, L. & Delaney, K. Odor-evoked calcium signals in dendrites of rat mitral cells. Proc. Natl Acad. Sci. USA 98, 1230–1234 (2001).
Barretto, R. P. J. et al. The neural representation of taste quality at the periphery. Nature 517, 373–U511 (2015).
Kim, Y. S. et al. Coupled activation of primary sensory neurons contributes to chronic pain. Neuron 91, 1085–1096 (2016).
Imamura, S. & Adams, J. C. Distribution of gentamicin in the guinea pig inner ear after local or systemic application. J. Assoc. Res. Otolaryngol. 4, 176–195 (2003).
Wang, Q. & Steyger, P. S. Trafficking of systemic fluorescent gentamicin into the cochlea and hair cells. J. Assoc. Res. Otolaryngol. 10, 205–219 (2009).
Tanoshima, R. et al. Analyses of adverse drug reactions-nationwide active surveillance network: Canadian Pharmacogenomics Network for Drug Safety Database. J. Clin. Pharmacol. 59, 356–363 (2019).
Du, X. et al. A hand-guided robotic drill for cochleostomy on human cadavers. Robot Surg. 5, 13–18 (2018).
Fishman, A. J., Moreno, L. E., Rivera, A. & Richter, C. P. CO2 laser fiber soft cochleostomy: development of a technique using human temporal bones and a guinea pig model. Laser Surg. Med. 42, 245–256 (2010).
Ren, D. D. & Chi, F. L. Experimental study on thermic effects, morphology and function of guinea pig cochlea: a comparison between the erbium: yttrium–aluminum–garnet laser and carbon dioxide laser. Laser Surg. Med. 40, 407–414 (2008).
Cipolla, M. J., Iyer, P., Dome, C., Welling, D. B. & Bush, M. L. Modification and comparison of minimally invasive cochleostomy techniques: a pilot study. Laryngoscope 122, 1142–1147 (2012).
Kiefer, J. et al. Conservation of low-frequency hearing in cochlear implantation. Acta Oto-Laryngol. 124, 272–280 (2004).
Dong, W. & Cooper, N. P. An experimental study into the acousto-mechanical effects of invading the cochlea. J. R. Soc. Interface 3, 561–571 (2006).
Cooper, N. P. & Rhode, W. S. Fast travelling waves, slow travelling waves and their interactions in experimental studies of apical cochlear mechanics. Audit Neurosci. 2, 289–299 (1996).
Ulfendahl, M., Khanna, S. M. & Flock, A. Effects of opening and resealing the cochlea on the mechanical response in the isolated temporal bone preparation. Hearing Res. 57, 31–37 (1991).
Alyono, J. C., Corrales, C. E., Huth, M. E., Blevins, N. H. & Ricci, A. J. Development and characterization of chemical cochleostomy in the guinea pig. Otolaryngol. Head. Neck Surg. 152, 1113–1118 (2015).
Kim, J. & Ricci, A.J. In vivo real-time imaging reveals megalin as the aminoglycoside gentamicin transporter into cochlea whose inhibition is otoprotective. Proc. Natl Acad. Sci. USA 119, e2117946119 (2022).
Lee, H. Y. et al. Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea. Proc. Natl Acad. Sci. USA 112, 3128–3133 (2015).
Nuttall, A. L., Dolan, D. F. & Avinash, G. Laser Doppler velocimetry of basilar membrane vibration. Hear. Res. 51, 203–213 (1991).
Ruggero, M. A. & Rich, N. C. Application of a commercially-manufactured Doppler-shift laser velocimeter to the measurement of basilar-membrane vibration. Hear. Res. 51, 215–230 (1991).
Ren, T. Longitudinal pattern of basilar membrane vibration in the sensitive cochlea. Proc. Natl Acad. Sci. USA 99, 17101–17106 (2002).
Ren, T. Reverse propagation of sound in the gerbil cochlea. Nat. Neurosci. 7, 333–334 (2004).
Dong, W. & Olson, E. S. Detection of cochlear amplification and its activation. Biophys. J. 105, 1067–1078 (2013).
Dallos, P. Response characteristics of mammalian cochlear hair cells. J. Neurosci. 5, 1591–1608 (1985).
Russell, I. J. & Sellick, P. M. Low-frequency characteristics of intracellularly recorded receptor potentials in guinea-pig cochlear hair cells. J. Physiol. 338, 179–206 (1983).
Liberman, M. C. Auditory-nerve response from cats raised in a low-noise chamber. J. Acoust. Soc. Am. 63, 442–455 (1978).
Lee, H. Y. et al. Two-dimensional cochlear micromechanics measured in vivo demonstrate radial tuning within the mouse organ of Corti. J. Neurosci. 36, 8160–8173 (2016).
Wang, R. K. & Nuttall, A. L. Phase-sensitive optical coherence tomography imaging of the tissue motion within the organ of Corti at a subnanometer scale: a preliminary study. J. Biomed. Opt. 15, 056005 (2010).
Jawadi, Z., Applegate, B. E. & Oghalai, J. S. Optical coherence tomography to measure sound-induced motions within the mouse organ of Corti in vivo. Methods Mol. Biol. 1427, 449–462 (2016).
Kim, J., Xia, A., Grillet, N., Applegate, B. E. & Oghalai, J. S. Osmotic stabilization prevents cochlear synaptopathy after blast trauma. Proc. Natl Acad. Sci. USA 115, E4853–E4860 (2018).
McGinley, M. J., Liberman, M. C., Bal, R. & Oertel, D. Generating synchrony from the asynchronous: compensation for cochlear traveling wave delays by the dendrites of individual brainstem neurons. J. Neurosci. 32, 9301–9311 (2012).
Marker, D.F., Tremblay, M.E., Lu, S.M., Majewska, A.K. & Gelbard, H.A. A thin-skull window technique for chronic two-photon in vivo imaging of murine microglia in models of neuroinflammation. J. Vis. Exp. 43, 2059 (2010).
Mancini, M. et al. Head and neck veins of the mouse. a magnetic resonance, micro computed tomography and high frequency color Doppler ultrasound study. PLoS ONE 10, e0129912 (2015).

A chemo-mechanical cochleostomy preserves hearing for the in vivo functional imaging of cochlear cells

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