3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling

Luca Pinton, Moustafa Khedr, Valentina M. Lionello, Shilpita Sarcar, Sara M. Maffioletti, Sumitava Dastidar, Elisa Negroni, SungWoo Choi, Noreen Khokhar, Anne Bigot, John R. Counsell, Andreia Sofia Bernardo, Peter S. Zammit, Francesco Saverio Tedesco

Published: 2023-02-14 DOI: 10.1038/s41596-022-00790-8

induced pluripotent stem cells

bioengineered skeletal muscles

3D hydrogels

muscle pathology

therapy modeling

AI 解读

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis

Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Tutorial: design and execution of CRISPR in vivo screens

Reproducible strategy for excisional skin-wound-healing studies in mice

CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

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Skeletal muscle is a complex tissue composed of multinucleated myofibers responsible for force generation that are supported by multiple cell types. Many severe and lethal disorders affect skeletal muscle; therefore, engineering models to reproduce such c
Introduction
Materials
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Timing
Anticipated results
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling

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