sample_prep_serum.nan
NAN KB, Mario Uchimiya, John Glushka, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
Disclaimer
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Abstract
This is a modified protocol for a protein precipitation method for plasma/serum samples. This protocol was originally proposed by:
Before start
Steps
Day-1/2
Thaw samples On ice or at 4°C
Add 600µL of 100% cold methanol to 300µL of samples On ice
- Use 1.5-mL Eppendorf tubes
- Keep methanol cold
On ice
Vortex the samples for 0h 0m 10s
Incubate the samples at -20°C for 0h 20m 0s
Centrifuge the samples at 4°C at 16000rcf for 0h 30m 0s
Transfer the supernatants to new 1.5-mL Eppendorf tubes
Dry the samples in a speed-vac concentrator
Day-2/2
Thaw the samples On ice or in 4°C
Add 600µL of the phosphate buffer to each sample
Vortex the samples at 4°C for 0h 10m 0s
Centrifuge the samples at 4°C for 0h 0m 10s
Transfer 580µL to 5-mm NMR tubes
