eDNA extraction with kaneka

We used the Kaneka Simple DNA Extraction Kit version 2 (Kaneka) for on-site DNA extraction from Sterivex. We injected 500 µl of Solution A of the kit into the Sterivex and shook it by hand for 1 min. We collected the Solution A buffer in the Sterivex by connecting the syringe with the connecting tube. We added 70 µl of Solution B into the collected Solution A buffer in a 1.5-ml microtube and centrifuged (~2,000  g ) with a portable centrifuge for 3 min. We collected 200 µl of the supernatant solution.