Y Maze Forced Alternation
Haley Geertsma
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5smq6c6
Abstract
This protocol is used to test mice in the Y Maze Forced Alternation behavioural test.
Steps
1.
Habituate mice in a separate room for 60 minutes prior to testing.
2.
Confirm testing room lighting is set to 60lux and EthoVision is set up properly.
3.
Place mice in Arm 1 (with either Arm 2 or 3 blocked, alternate between mice) and record their movement for 5 minutes.
4.
After testing, place the mouse back into the home cage and clean the Y maze. Leave the mouse in its home cage for 30 minutes.
5.
Remove the blockage so all 3 arms are open and place the mouse back into Arm 1. Record their movement for 5 minutes.
6.
Once testing is complete, return mouse to its home cage and clean the Y maze.
