Worm Freezing Buffer
Adrien Assie, Buck Samuel
Disclaimer
Original protocol found on wormbook from Brener et al. 1974
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
Worm Freezing buffer recipe according to Brenner, S. (1974). Genetics 77 , 71. Found on wormbook see external link
Steps
150mLof 100% volume glycerol
25mL of 1Molarity (M) Potassium Phosphate Buffer, pH 6.0
2.85gof NaCl
500mLof M9 Buffer
Adjust deionized water to1000mL
Autoclave
