Tween 60-esculin Agar (TE slant)

Amy Gladfelter

Published: 2023-06-02 DOI: 10.17504/protocols.io.yxmvm73e5v3p/v1

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Abstract

This media is used to determine ability of Malassezia species to hydrolyzeesculin and utilize Tween 60.

Before start

Attachments

Malassezia media_1.pdf

Steps

1.

peptone 10g

2.

glucose 10g

3.

yeast extract 2g

4.

ferric ammonium citrate 0.5g

5.

esculin 1g

6.

agar 15g

7.

Tween 60 5mL

8.

dH₂O 1L

9.

Sterilize by autoclaving.

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