Tutorial: design and execution of CRISPR in vivo screens

Christian J. Braun, Dieter Saur, Roland Rad, Andrés Carbonell Adames

Published: 2022-07-14 DOI: 10.1038/s41596-022-00700-y

CRISPR in vivo screening

mouse model

technical execution

phenotype interrogation

genetic interaction screening

AI 解读

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Here we provide a detailed tutorial on CRISPR invivo screening. Using the mouse as the model organism, we introduce a range of CRISPR tools and applications, delineate general considerations for ‘transplantation-based’ or ‘direct
Main
General considerations for invivo CRISPR screening
Design and execution of transplantation screens
Screening phenotypes in transplantation screens
Design and execution of direct invivo CRISPR screens
Deriving data from an invivo screen
Limitations and troubleshooting
Novel screening applications
Conclusions and future outlooks
- References
References
ACKNOWLEDGMENTS

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Tutorial: design and execution of CRISPR in vivo screens

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