Transformation of E. coli with plasmid

Wash cuvette with 100% (v/v) ethanol and UV in the hood for 0h 20m 0s

Pre-thaw electrocompetent cells and plasmid on wet ice for 0h 10m 0s

Add 1µL of plasmid DNA to 50µL electrocompetent cells

Transfer cell-DNA mixture into the cuvette and add equal amount (50µL ) of MilliQ

Let the cuvette sit in wet ice for 0h 20m 0s

Place cuvette in BioRad pulser and pulse at 2.5 keV

Within 0h 0m 40s add 100µL of 2X LB to the cuvette

Place cuvette in an incubator at 37°C for 0h 30m 0s

Then use a bent pipette tip to spread the cuvette's contents onto a plate made with LB agar and the appropriate antibiotics. Spread until plate is dry

Place the plate in an incubator at 37°C

Make glycerol stocks of transformed cells and store for future use