Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq

Yusheng Liu, Yiwei Zhang, Jiaqiang Wang, Falong Lu

Published: 2022-07-12 DOI: 10.1038/s41596-022-00704-8

PAIso-seq

poly(A) tails

PacBio sequencing

RNA isoform

single-oocyte sensitivity

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Poly(A) tails are added to the 3′ ends of most mRNAs in a non-templated manner and play essential roles in post-transcriptional regulation, including mRNA export, stability and translation. Measuring poly(A) tails is critical for understanding the
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ACKNOWLEDGMENTS

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Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq

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