Taxon group: Non-larval Arthropods (TSS1)

Lyndall Pereira da Conceicoa, Olga Sivell, Laura Sivess, Chris Fletcher, Gavin R. Broad, Liam Crowley, Inez Januszczak

Published: 2022-05-04 DOI: 10.17504/protocols.io.81wgb66jylpk/v1

Abstract

This is part of the collection "DToL Taxon-specific Standard Operating Procedure (SOP) for the Terrestrial and Freshwater Arthropods Working Group". The SOP collection contains guidance on how to process the various terrestrial and freshwater arthropod taxa within the scope of the Darwin Tree of Life project. The guidance specifically refers to the tissue samples needed for DNA barcoding (which takes place at the Natural History Museum (NHM)) and outlines the dissected tissues required for whole genome sequencing (WGS), which takes place at the Wellcome Sanger Institute . Every specimen is submitted for DNA barcoding first before potentially being sent to the Wellcome Sanger institute.

TSS1 = Taxon Specific SOP for Non-larval Arthropods

Definition : Adult terrestrial and freshwater arthropods and immature life stages of hemimetabolous taxa; over 5 mm body length.

Including : Coleoptera, Hymenoptera, Diptera, Lepidoptera, other insect orders (Blattodea, Dermaptera, Ephemeroptera, Hemiptera, Mecoptera, Megaloptera, Neuroptera, Odonata, Orthoptera, Plecoptera, Raphidioptera, Trichoptera), Amphipoda, Isopoda, Diplopoda, Chilopoda, Araneae, Opiliones, Scorpiones.

Excluding : Specimens smaller than 5mm

Pictorial dissection guides available.

See the Guidelines for important details and checklist.

Steps

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