TS Spurrs - Cell Monolayer on Thermanox coverslips (TM - 013)
sandra.crameri
Published: 2023-05-09 DOI: 10.17504/protocols.io.n92ldpk17l5b/v1
Abstract
Processing a cell monolayer on Thermanox coverslips to Spurrs resin block for EM.
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
Conventional - with reduced times for monlayer thickness
2.
2.5%% volume 0.1Molarity (M) Sorenson's phosphate buffer 07.2 300 mosmol/kg0h 20m 0s.
3.
Wash buffer 0h 5m 0s
4.
1Mass / % volume 0h 30m 0s
5.
70% volume 0h 10m 0s
6.
95% volume 0h 5m 0s
7.
100% volume 0h 5m 0s
8.
100% volume 0h 5m 0s
9.
100% volume Spurrs:100% volume 0h 30m 0s
10.
100% volume Spurrs0h 30m 0s
11.
100% volume Spurrs0h 30m 0s
12.
Oven polymerise 65°C
