Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way
Yogendra Verma, Hanqin Li, Dirk Hockemeyer, Frank Soldner
Published: 2022-09-06 DOI: 10.17504/protocols.io.b4mmqu46
Abstract
This collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way. This collection follows nucleofection of hPSCs as described in detail in the collection "Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs;" dx.doi.org/10.17504/protocols.io.b4qnqvve
Collection overview
Seeding nucleofected hPSCs in 96-well plates using limited dilution
Duplicating 96-well plate-cultured hPSCs clones
Subcloning of genotype-confirmed hPSCs clones
General notes
- Throughout these protocols, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.
