Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins

Yi Yang, Renmei Liu, Jing Yao, Siyu Zhou, Jing Yang, Yaqiang Zhang, Xiaoyan Yang, Leshi Li, Yunbin Zhang, Yingping Zhuang, Xianjun Chen

Published: 2023-11-30 DOI: 10.1038/s41596-023-00920-w

RNA-binding proteins

Photoswitchable RBPs

Spatiotemporal control

Optogenetic

CRISPR-Cas functions

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–8 and Supplementary Note

Reporting Summary

Supplementary Data 1

Statistical source data for Supplementary Figs. 5 and 7

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis

Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Tutorial: design and execution of CRISPR in vivo screens

Reproducible strategy for excisional skin-wound-healing studies in mice

CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

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Supplementary information

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Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins

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