SmartSeq cecilia, Suzie Alarcon, Alessandro Sette Published: 2021-12-02 DOI: 10.17504/protocols.io.bxr6pm9e SmartSeq Custom QXT ASAPCRN AI 解读 浏览 1162 Abstract This protocol details the collection of methods of SmartSeq, Part 1: SmartSeq and Part 2: Custom QXT. Attachments d2bybg4xf.docx d2bxbg4xf.docx Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution Isotropic imaging across spatial scales with axially swept light-sheet microscopy Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster 查看全部 Slide Preparation and Transect Counting of Paleoecological Microfossils - SPaTCoPM Tissue Cyclic Immunofluorescence (t-CyCIF) version 3 Protocol for performing PINK1 siRNA knockdown in mouse embryonic fibroblasts (MEFs) ASAPCRN Plasmid Sequence Analysis from Long Reads LAMP Protocol for Entomopathogenic Serratia spp. in Insect Frass Frass DNA extraction LAMP Serratia Insect Diagnostic Gene knockout strategy Recombineering counterselection selection markers genome engineering synthetic biology tetA immunoblotting (JMS protocol) ASAPCRN Chloroquine agarose gel Donor Selection Criteria for Adipose, Liver & Blood Procurement -- University of Minnesota Human TMC Adipose Human SenNet criteria UMN UMinnesota University of Minnesota Niedernhofer Donor criteria CPT Processing CPT processing ASAPCRN 查看全部 Using Mothur to Determine Bacterial Community Composition and Structure in 16S Ribosomal RNA Datasets 16S rRNA microbiome OTUs Validation of a Novel, Flash-Freezing Method: Aluminum Platform DNA fresh tissue immunofluorescence liver pancreas RNA Culturing and Mating of Aspergillus fumigatus ascospores Aspergillus fumigatus cleistothecia mating sexual reproduction Small Interfering RNA Delivery Into Primary Human Natural Killer Cells for Functional Gene Analyses cell therapy gene function knockdown NK cells siRNA An In Vivo Mouse Model for Chronic Inflammation–Induced Immune Suppression: A “Factory” for Myeloid-Derived Suppressor Cells (MDSCs) chronic inflammation immune suppression M-MDSCs myeloid cells PMN-MDSCs Purification of Adeno-Associated Viral Vector Serotype 9 Using Ceramic Hydroxyapatite Chromatography and its Analysis AAV ceramic hydroxyapatite chromatography purification TFF UniLectin, A One-Stop-Shop to Explore and Study Carbohydrate-Binding Proteins carbohydrate glycan-binding lectin structural biology Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples cell viability FRET GEPIIs K+ potassium ions recombinant protein serum K+ Mouse Models of Sepsis cecal slurry sepsis CLP microbial sepsis mouse models sepsis Three-Dimensional Vessels-on-a-Chip Based on hiPSC-derived Vascular Endothelial and Smooth Muscle Cells differentiation endothelial cells human induced pluripotent stem cells neural crest vascular smooth muscle cells vessels-on-a-chip 查看全部
