SmartSeq cecilia, Suzie Alarcon, Alessandro Sette Published: 2021-12-02 DOI: 10.17504/protocols.io.bxr6pm9e SmartSeq Custom QXT ASAPCRN AI 解读 浏览 1131 Abstract This protocol details the collection of methods of SmartSeq, Part 1: SmartSeq and Part 2: Custom QXT. Attachments d2bybg4xf.docx d2bxbg4xf.docx Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq Generation of heart-forming organoids from human pluripotent stem cells Design and fabrication of wearable electronic textiles using twisted fiber-based threads Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo Chromatin accessibility profiling by ATAC-seq Deoxyfluorination of phenols for chemoselective18F-labeling of peptides Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening 查看全部 In-vitro GCase Activity Assay ASAPCRN GCase GCase activity Isolation of NeuN+ cells from brain tissue (for CUT and RUN) ASAPCRN URMC TriState SenNet Mouse Lung Digestion cellular senescence lung tissue Dissociation Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater Norovirus Oxford Nanopore Technologies Long Amplicon Wastewater Sequencing Assessment of prepulse inhibition (PPI) of the acoustic startle reflex in rodents Histology, Immunohistochemistry and Imaging ASAPCRN Differentiation NPCs to Dopaminergic/Midbrain Neurons differentiation dxn NPC Dopaminergic Midbrain Neurons ASAPCRN LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation ASAPCRN Tissue collection Characterizing spatial and temporal properties of ER-phagy receptors ASAPCRN 查看全部 Generation of 3D Tumor Spheroids with Encapsulating Basement Membranes for Invasion Studies spheroid basement membrane invasion 3D-model Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation 3Rs replacement reduction refinement automation genotyping molecular biology mouse model PCR reproducibility Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions fungal genomics maize plant genomics plant-microbe interactions unique molecular identifiers Ustilago maydis virulence factors ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Murine Norovirus: Additional Protocols for Basic and Antiviral Studies baculovirus expression circular polymerase extension reaction murine norovirus neonatal infections tuft cell-enriched murine intestinal enteroid culture Analysis of Genetic Ancestry from NGS Data Using EthSEQ ancestry analysis NGS SNPs Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Genomic Epidemiology Analysis of Infectious Disease Outbreaks Using TransPhylo genomic epidemiology infectious disease outbreak phylogenetics transmission analysis 查看全部
