Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron

A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat.

Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)

Step 2: Ganglia dissociation and single-cell sorting

Step 3: Single-cell sequencing and analysis