Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system Hao Wu, Ling-Ling Chen, Siqi Li Published: 2022-07-12 DOI: 10.1038/s41596-022-00715-5 circRNA screening RfxCas13d BSJ-gRNA functional annotation CRISPR–Cas13 AI 解读 浏览 1520 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Metagenome-assembled genome extraction and analysis from microbiomes using KBase Selected ion flow tube mass spectrometry for targeted analysis of volatile organic compounds in human breath Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools Development of RNA G-quadruplex (rG4)-targetingl-RNA aptamers by rG4-SELEX Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales Genetic in vivo engineering of human T lymphocytes in mouse models Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness 查看全部 Non-guided neural organoids differentiation ASAPCRN Non-Photochemical Quenching (NPQ) Relaxation Analysis by CF imager FluorCam FC800-331 and FluorCam 7 Software protocols.io academic and non-profit contract Transformation Protocol quick ligation transformation comp cells Transformation Competent cells Participant Registration Form For Mapping the Early Childhood Gut Across Ancestry, Geography, and Environment (Gut-AGE) S-2 SOIL PROCESSING soil processing QUBIT FLUOROMETER SAMPLE LYSIS EXTRACTION Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay ASAPCRN LRRK2 Rab12 Co-IP Immunoprecipitation Co-immunoprecipitation Very low-density lipoprotein receptor (VLDLR)-C-tag purification from HEK293E cells ASAPCRN Immunohistochemistry for brain sections ASAPCRN immunohistochemistry brain mouse Electrochemical analysis of laser-inscribed graphene electrodes using cyclic voltammetry (ferri/ferrocyanide redox couple) 查看全部 Fabrication of Human Skin Equivalents Using Decellularized Extracellular Matrix biomaterials extracellular matrix in vitro models skin tissue engineering Cryogel-based Injectable 3D Microcarrier Co-culture for Support of Hematopoietic Progenitor Niches biomaterial bone marrow cryogel hematopoietic injectable scaffold stem cell niche tissue engineering A Rapid and Universal Workflow for Label-Free-Quantitation-Based Proteomic and Phosphoproteomic Studies in Cereals maize phosphopeptide enrichment phosphoproteome protein proteome wheat Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Analysis of Tomato Post-Harvest Properties: Fruit Color, Shelf Life, and Fungal Susceptibility Botrytis fruit color fruit quality fruit storage fungal susceptibility post-harvest analysis shelf life Rapid Synthesis of Nucleoside Triphosphates and Analogues cyclotriphosphate non-hydrolyzable nucleotide analogues nucleoside 5′-triphosphate phosphoramidite phosphorylation Generation of 3D Tumor Spheroids with Encapsulating Basement Membranes for Invasion Studies spheroid basement membrane invasion 3D-model Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction NDEx: Accessing Network Models and Streamlining Network Biology Workflows Cytoscape enrichment analysis JavaScript NDEx neighborhood query network biology network models pathway python R REST API subnetwork systems biology QIIME 2 Enables Comprehensive End-to-End Analysis of Diverse Microbiome Data and Comparative Studies with Publicly Available Data bioinformatics metagenomics microbiome QIIME 2 Qiita 查看全部