Scalable single-cell profiling of chromatin modifications with sciCUT&Tag

Kami Ahmad, Steven Henikoff, Samuel S. Minot, Derek H. Janssens, Jacob E. Greene, Steven J. Wu, Christine A. Codomo, Scott N. Furlan

Published: 2023-11-07 DOI: 10.1038/s41596-023-00905-9

single-cell chromatin profiling

Supplementary information

Supplementary Information

Supplementary Methods and Figs. 1 and 2.

All the barcoded Tn5 s5 and s7 and universal MErev adapter sequences are shown in the first tab, the barcoded i5 and i7 primer sequences in the second tab and the custom primer sequences used for illumine sequencing in the third tab.

Supplementary Table 2

Layout of the 96-well sci-pA–Tn5 plate

Supplementary Table 3

Layout of the 384-well i5 and i7 primer plate for dispensing on the ICell8 instrument

Supplementary Table 4

An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name prefixes based on the s5 and s7 adapter sequence

Supplementary Table 5

An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name suffixes based on the i5 and i7 adapter sequence

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

References

Supplementary information

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Scalable single-cell profiling of chromatin modifications with sciCUT&Tag

Supplementary information

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