Scalable single-cell profiling of chromatin modifications with sciCUT&Tag
Kami Ahmad, Steven Henikoff, Samuel S. Minot, Derek H. Janssens, Jacob E. Greene, Steven J. Wu, Christine A. Codomo, Scott N. Furlan
histone modifications
combinatorial indexing
single-cell chromatin profiling
CUT&Tag
nanowell dispenser




































Supplementary information
Supplementary Information
Supplementary Methods and Figs. 1 and 2.
Reporting Summary
Supplementary Table 1
All the barcoded Tn5 s5 and s7 and universal MErev adapter sequences are shown in the first tab, the barcoded i5 and i7 primer sequences in the second tab and the custom primer sequences used for illumine sequencing in the third tab.
Supplementary Table 2
Layout of the 96-well sci-pA–Tn5 plate
Supplementary Table 3
Layout of the 384-well i5 and i7 primer plate for dispensing on the ICell8 instrument
Supplementary Table 4
An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name prefixes based on the s5 and s7 adapter sequence
Supplementary Table 5
An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name suffixes based on the i5 and i7 adapter sequence