SDS-PAGE
Alexander Röntgen
Published: 2023-11-02 DOI: 10.17504/protocols.io.4r3l22er4l1y/v1
Abstract
Protocol for running an SDS-PAGE.
Steps
Sample preparation
1.
Dilute NuPAGE Sample Buffer 4X Concentrate into protein samples
2.
Heat for 10 min at 95 °C
Gel
3.
Run gel at ~150 V for 35 min
4.
Incubate in staining solution until bands appear
5.
Image using ChemiDoc MP Imaging system
