Reprogramming fibroblast into human iBlastoids

Xiaodong Liu, Jia Ping Tan, Jose M. Polo

Published: 2024-04-17 DOI: 10.1038/s41596-024-00984-2

in vitro model

iBlastoids

somatic reprogramming

fibroblast

early embryogenesis

AI 解读

Growing and making nano- and microcrystals

Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues

Isocyanide-based multi-component reactions for carrier-free and carrier-bound covalent immobilization of enzymes

Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Stabilization and structure determination of integral membrane proteins by termini restraining

Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy

Multiplexed single-cell analysis of organoid signaling networks

Chromatin accessibility profiling by ATAC-seq

Publisher Correction: A practical guide to large-scale docking

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References

Fu, J., Warmflash, A. & Lutolf, M. P. Stem-cell-based embryo models for fundamental research and translation. Nat. Mater. 20, 132–144 (2020).
Rossant, J. & Tam, P. P. L. Opportunities and challenges with stem cell-based embryo models. Stem Cell Rep. 16, 1031–1038 (2021).
Posfai, E., Lanner, F., Mulas, C. & Leitch, H. G. All models are wrong, but some are useful: establishing standards for stem cell-based embryo models. Stem Cell Rep. 16, 1117–1141 (2021).
Rivron, N. C. et al. Blastocyst-like structures generated solely from stem cells. Nature 557, 106–111 (2018).
Sozen, B. et al. Self-organization of mouse stem cells into an extended potential blastoid. Dev. Cell 51, 698–712.e8 (2019).
Li, R. et al. Generation of blastocyst-like structures from mouse embryonic and adult cell cultures. Cell 179, 687–702.e18 (2019).
Harrison, S. E., Sozen, B., Christodoulou, N., Kyprianou, C. & Zernicka-Goetz, M. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science 356, eaal1810 (2017).
Zhang, S. et al. Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat. Commun. 10, 496 (2019).
Sozen, B. et al. Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures. Nat. Cell Biol. 20, 979–989 (2018).
Amadei, G. et al. Inducible stem cell-derived embryos capture mouse morphogenetic events in vitro. Dev. Cell 56, 366–382.e9 (2021).
Xu, Y. et al. Derivation of totipotent-like stem cells with blastocyst-like structure forming potential. Cell Res 32, 513–529 (2022).
Shen, H. et al. Mouse totipotent stem cells captured and maintained through spliceosomal repression. Cell 184, 2843–2859.e20 (2021).
Hu, Y. et al. Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature 617, 792–797 (2022).
Amadei, G. et al. Embryo model completes gastrulation to neurulation and organogenesis. Nature 610, 143–153 (2022).
Tarazi, S. et al. Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs. Cell 185, 3290–3306.e25 (2022).
Liu, X. et al. Reprogramming roadmap reveals route to human induced trophoblast stem cells. Nature 586, 101–107 (2020).
Tan, J. P., Liu, X. & Polo, J. M. Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts. Nat. Protoc. 17, 2739–2759 (2022).
Liu, X. et al. Modelling human blastocysts by reprogramming fibroblasts into iBlastoids. Nature 591, 627–632 (2021).
Yu, L. et al. Blastocyst-like structures generated from human pluripotent stem cells. Nature 591, 620–626 (2021).
Kagawa, H. et al. Human blastoids model blastocyst development and implantation. Nature 601, 600–605 (2022).
Yanagida, A. et al. Naive stem cell blastocyst model captures human embryo lineage segregation. Cell Stem Cell 28, 1016–1022.e4 (2021).
Io, S. et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell 28, 1023–1039.e13 (2021).
Guo, G. et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28, 1040–1056.e6 (2021).
Fan, Y. et al. Generation of human blastocyst-like structures from pluripotent stem cells. Cell Discov. 7, 81 (2021).
Sozen, B. et al. Reconstructing aspects of human embryogenesis with pluripotent stem cells. Nat. Commun. 12, 5550 (2021).
Mazid, M. A. et al. Rolling back human pluripotent stem cells to an eight-cell embryo-like stage. Nature 605, 315–324 (2022).
Yu, X. et al. Recapitulating early human development with 8C-like cells. Cell Rep. 39, 110994 (2022).
Taubenschmid-Stowers, J. et al. 8C-like cells capture the human zygotic genome activation program in vitro. Cell Stem Cell 29, 449–459.e6 (2022).
Yu, L. et al. Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk. Cell Stem Cell 30, 1246–1261.e9 (2023).
Yu, J. et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797–801 (2009).
Warren, L. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7, 618–630 (2010).
Clark, A. T. et al. Human embryo research, stem cell-derived embryo models and in vitro gametogenesis: considerations leading to the revised ISSCR guidelines. Stem Cell Rep. 16, 1416–1424 (2021).
Lovell-Badge, R. et al. ISSCR guidelines for stem cell research and clinical translation: the 2021 update. Stem Cell Rep. 16, 1398–1408 (2021).
Shahbazi, M. N. et al. Self-organization of the human embryo in the absence of maternal tissues. Nat. Cell Biol. 18, 700–708 (2016).
Deglincerti, A. et al. Self-organization of the in vitro attached human embryo. Nature 533, 251–254 (2016).

Reprogramming fibroblast into human iBlastoids

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