Reprogramming fibroblast into human iBlastoids Xiaodong Liu, Jia Ping Tan, Jose M. Polo Published: 2024-04-17 DOI: 10.1038/s41596-024-00984-2 in vitro model iBlastoids somatic reprogramming fibroblast early embryogenesis AI 解读 浏览 1372 PDFMetadataExtended Supplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Preparation of single-cell suspensions of mouse glomeruli for high-throughput analysis cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements) Genome-wide pooled CRISPR screening in neurospheres Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Design and fabrication of wearable electronic textiles using twisted fiber-based threads Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells Scanorama: integrating large and diverse single-cell transcriptomic datasets 查看全部 Concentration and nucleic acid extraction of viruses from aggregated airplane waste wastewater-based epidemiology WBE aggregated airplane waste airplane wastewater wastewater viral concentration viromics metagenomics Enhancing the growth of coral microfragments using the 'Green Film' Husbandry Method microfragmentation microfragment coral stony coral aquarium husbandry aquaculture coral culture DNA extraction from recently fertilised Atlantic salmon embryos for use in microsatellite validation of triploidy DNA extraction DNA quality and quantity assessments Microsatellites validation assessment Egg Tiploid Salmon How to grow wheat in controlled and semi-controlled environment Wheat Germination Transplanting Threshing Vernalisation Preparation of 1M calcium chloride solution (CaCl2) ngm worm c.elegans c elegans elegans caenorhabditis media medium calcium chloride RNA-Stable Isotope Probing Stable isotope probing SIP RNA Ultracentrifugation Density gradient Resuspension and concentration of iron chloride precipitated viruses from seawater for HMW DNA extraction Patch-Seq Recording and Extraction Detailed Protocol Patch-Seq patch seq recording electrophysiology neuron PF0301 patching extraction extracting Confirmation of Gene Knockdown with RT-qPCR ASAPCRN DTI preprocessing, statistical and cluster analysis diffusion tensor imaging 查看全部 Exploring Non-Coding RNAs in RNAcentral Galaxy ncRNA non-coding RNA RNAcentral RNA-seq Purification of SRSF1 from E. coli for Biophysical and Biochemical Assays intrinsically disordered proteins NMR phase separation phosphorylation RNA splicing SR protein SRSF1 Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors Cas9 CRISPR double-stranded DNA donors fluorescent protein genome engineering homology-directed repair tissue culture cells Engineering Ligand and Receptor Pairs with LIPSTIC to Track Cell-Cell Interactions cell-cell interactions enzymatic labeling immune interactions LIPSTIC Streptozotocin-Induced Diabetic Models in Mice and Rats high-fat hyperglycemia insulin deficiency insulin resistance insulitis mouse or rat nicotinamide streptozotocin type 1 diabetes mellitus type 2 diabetes mellitus Solid-Supported Membrane (SSM)-Based Electrophysiology Assays Using Surface Electrogenic Event Reader Technology (SURFE²R) in Early Drug Discovery drug discovery high-throughput screening solid-supported membrane (SSM)-based electrophysiology SURFE²R assay Cassava Metabolomics and Starch Quality chemotypes Manihot esculenta metabolite classification metabolite profiling metabolomics Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis The COPILOT Raw Illumina Genotyping QC Protocol docker genotyping GWAS Illumina QC pipeline The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses AIM epitopes infectious diseases lyophilization SARS-CoV-2 T cell 查看全部
